Ulatowska Beata, Gospodarek Eugenia
Katedra i Zakład Mikrobiologii Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu.
Med Dosw Mikrobiol. 2006;58(1):53-8.
For over fifty years Serratia spp. rods have increasingly been isolated from clinical specimens. The isolation has arisen mainly from hospitalised patients. The development of Serratia spp. infections is associated with predisposing factors of patients, occurrence in both natural settings and within the hospital environment and the virulence factors of bacteria. CDM medium with casein sodium salt, gelatine and Skim Milk were used to detect proteolytic properties. Over 95% of analysed strains produced gelatinase. Casein sodium salt was hydrolysed by 89,6% to 93,1% of strains depending on cultivation temperature. Skim Milk was hydrolysed by 78,5% to 84,7% of analysed Serratia spp. strains. Haemolytic properties of Serratia spp. rods were checked on a solid medium with human and sheep red blood cells at 22, 30, and 37 degrees C. The haemolysis of human erythrocytes at 37 degrees C was detected most frequently at 38,2%. There were no significant differences identified when cultivation temperature was observed.
五十多年来，沙雷氏菌属杆菌越来越多地从临床标本中分离出来。这种分离主要来自住院患者。沙雷氏菌属感染的发生与患者的易感因素、在自然环境和医院环境中的出现情况以及细菌的毒力因子有关。使用含有酪蛋白钠盐、明胶和脱脂牛奶的CDM培养基来检测蛋白水解特性。超过95%的分析菌株产生明胶酶。根据培养温度，89.6%至93.1%的菌株可水解酪蛋白钠盐。78.5%至84.7%的分析沙雷氏菌属菌株可水解脱脂牛奶。在含有人类和绵羊红细胞的固体培养基上，于22℃、30℃和37℃检测沙雷氏菌属杆菌的溶血特性。在37℃时，人类红细胞的溶血现象最常被检测到，发生率为38.2%。观察培养温度时未发现显著差异。
