Furukawa T, Fioretti A, Plotkin S
J Virol. 1973 Jun;11(6):991-7. doi: 10.1128/JVI.11.6.991-997.1973.
In high-multiplicity infection of human fibroblasts, human cytomegalovirus of WI-38 human diploid cells produced early cell rounding 6 to 24 h after inoculation. This early cell rounding was caused only by inoculation with infectious virions. Inhibitors of protein synthesis, but not DNA inhibitors, prevented this cytopathic effect. Apparently, a new protein is synthesized in infected fibroblasts from about 2 h postinoculation. Infectivity of cell-associated and supernatant infectious virus reached maximal levels at 5 to 7 and 10 days postinoculation, respectively. Synthesis of DNA, infectious virus, complement-fixing antigen, and precipitin antigen all began between 24 and 48 h, with the bulk of synthesis occurring 48 to 96 h postinoculation.
在人成纤维细胞的高复数感染中，WI-38人二倍体细胞的人巨细胞病毒在接种后6至24小时引起早期细胞变圆。这种早期细胞变圆仅由接种感染性病毒粒子引起。蛋白质合成抑制剂而非DNA抑制剂可阻止这种细胞病变效应。显然，在接种后约2小时，感染的成纤维细胞中会合成一种新蛋白质。细胞相关和上清液感染性病毒的感染性分别在接种后5至7天和10天达到最高水平。DNA、感染性病毒、补体结合抗原和沉淀素抗原的合成均在24至48小时之间开始，大部分合成发生在接种后48至96小时。
