Hsu Chin-Yuan
Department of Life Science, Chang Gung University, Tao-Yuan, Taiwan, China.
Biol Res. 2006;39(2):281-8. doi: 10.4067/s0716-97602006000200010. Epub 2006 Jul 25.
Free radicals induce numerous diseases by lipid peroxidation, protein peroxidation, and DNA damage. It has been reported that numerous plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum aviculare L. (Polygonaceae) has antioxidant activity is unknown. In this study, dried Polygonum aviculare L. was extracted by ethanol, and the extract was lyophilized. The antioxidant activities of extract powder were examined by free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays. The results show that the IC50 value of Polygonum aviculare L. extract is 50 microg/ml in free radical scavenging assays, 0.8 microg/ml in superoxide radical scavenging assays, and 15 microg/ml in lipid peroxidation assays, respectively. Furthermore, Polygonum aviculare L. extract has DNA protective effect in hydroxyl radical-induced DNA strand scission assays. The total phenolics and flavonoid content of extract is 677.4 +/- 62.7 microg/g and 112.7 +/- 13 microg/g. The results indicate that Polygonum aviculare L. extract clearly has antioxidant effects.
自由基通过脂质过氧化、蛋白质过氧化和DNA损伤诱发多种疾病。据报道，许多植物提取物具有清除自由基的抗氧化活性。扁蓄（蓼科）是否具有抗氧化活性尚不清楚。在本研究中，扁蓄干燥物用乙醇提取，提取物经冻干处理。提取物粉末的抗氧化活性通过自由基清除试验、超氧阴离子自由基清除试验、脂质过氧化试验和羟自由基诱导的DNA链断裂试验进行检测。结果表明，扁蓄提取物在自由基清除试验中的IC50值为50微克/毫升，在超氧阴离子自由基清除试验中为0.8微克/毫升，在脂质过氧化试验中为15微克/毫升。此外，扁蓄提取物在羟自由基诱导的DNA链断裂试验中具有DNA保护作用。提取物中总酚和黄酮类化合物的含量分别为677.4±62.7微克/克和112.7±13微克/克。结果表明，扁蓄提取物具有明显的抗氧化作用。
