Morillo Jose Antonio, Aguilera Margarita, Ramos-Cormenzana Alberto, Monteoliva-Sánchez Mercedes
Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, Granada 18071, Spain.
Curr Microbiol. 2006 Sep;53(3):189-93. doi: 10.1007/s00284-005-0438-7. Epub 2006 Jul 27.
The present study investigated the use of two-phase olive mill waste (TPOMW) as substrate for the production of exopolysaccharide (EPS) by the endospore-forming bacilli Paenibacillus jamilae. This microorganism was able to grow and produce EPS in aqueous extracts of TPOMW as a unique source of carbon. The effects of substrate concentration and the addition of inorganic nutrients were investigated. Maximal polymer yield in 100-ml batch-culture experiments (2 g l(-1)) was obtained in cultures prepared with an aqueous extract of 20% TPOMW (w/v). An inhibitory effect was observed on growth and EPS production when TPOMW concentration was increased. Nutrient supplementation (nitrate, phosphate, and other inorganic nutrients) did not increase yield. Finally, an adsorption experiment of Pb (II), Cd (II), Cu (II), Zn (II), Co (II), and Ni (II) by EPS is reported. Lead was preferentially complexed by the polymer, with a maximal uptake of 230 mg/g EPS.
本研究调查了利用两相橄榄果渣废弃物（TPOMW）作为产芽孢杆菌杰米拉类芽孢杆菌生产胞外多糖（EPS）的底物。这种微生物能够在TPOMW水提物中生长并产生EPS，TPOMW水提物是唯一的碳源。研究了底物浓度和无机营养添加的影响。在100毫升分批培养实验中（2克/升），用20%（w/v）的TPOMW水提物制备的培养物中获得了最大聚合物产量。当TPOMW浓度增加时，观察到对生长和EPS产生有抑制作用。添加营养物质（硝酸盐、磷酸盐和其他无机营养物）并未提高产量。最后，报道了EPS对Pb（II）、Cd（II）、Cu（II）、Zn（II）、Co（II）和Ni（II）的吸附实验。铅优先与聚合物络合，最大吸附量为230毫克/克EPS。
