Aguilera Margarita, Quesada Maria Teresa, Del Aguila Víctor Guerra, Morillo José Antonio, Rivadeneyra Maria Angustias, Ramos-Cormenzana Alberto, Monteoliva-Sánchez Mercedes
Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, 18071 Granada, Spain.
Bioresour Technol. 2008 Sep;99(13):5640-4. doi: 10.1016/j.biortech.2007.10.032. Epub 2007 Dec 3.
A total of 10 bacterial strains were isolated from a compost of corn treated with olive mill wastewaters (OMW) and selected by their capacity to synthesize exopolysaccharides (EPS). Morphological, physiological, biochemical and nutritional tests were used for a phenotypic study. A numerical analysis showed that all strains were 90% similar to each other. A DNA-DNA hybridization assay confirmed that all the strains belonged to Paenibacillus jamilae species. All the characterized strains were able to produce EPS growing on OMW batch cultures. The strain which was able to produce the highest EPS yield was chosen to perform an assay for testing its putative detoxifying activity, and it showed to reduce more than half the toxic capacity of the OMW. The results presented in this study, indicated the possible perspectives for using these bacterial strains to produce EPS and contribute to the bioremediation of the waste waters that are produced in the olive oil elaboration process.
从用橄榄油厂废水（OMW）处理过的玉米堆肥中总共分离出10株细菌菌株，并根据它们合成胞外多糖（EPS）的能力进行筛选。形态学、生理学、生化和营养测试用于表型研究。数值分析表明，所有菌株彼此相似度达90%。DNA-DNA杂交分析证实所有菌株均属于Jamilae类芽孢杆菌种。所有已鉴定的菌株在OMW分批培养中生长时都能够产生EPS。选择能够产生最高EPS产量的菌株进行测定，以测试其假定的解毒活性，结果表明该菌株可将OMW的毒性降低一半以上。本研究给出的结果表明，利用这些细菌菌株生产EPS并有助于橄榄油加工过程中产生的废水进行生物修复具有潜在的可能性。
