Klausen B, Evans R T, Ramamurthy N S, Golub L M, Sfintescu C, Lee J Y, Bedi G, Zambon J J, Genco R J
Department of Microbiology, Royal Dental College, Copenhagen.
Oral Microbiol Immunol. 1991 Aug;6(4):193-201. doi: 10.1111/j.1399-302x.1991.tb00477.x.
Bacteroides gingivalis is associated with various forms of periodontal disease. To assess the role of the immune response in modulating B. gingivalis-associated periodontal disease, the effect of immunization of B. gingivalis-induced periodontal bone loss was evaluated in gnotobiotic rats. Male Sprague-Dawley rats immunized with various doses of whole cells or sham-immunized with incomplete Freund's adjuvant were monoinfected with B. gingivalis in carboxymethylcellulose by gavage. Two additional groups served as either sham-immunized or untreated germ-free controls. Forty-two days after infection, all rats were killed, periodontal bone level was assessed morphometrically and radiographically, and gingival proteinase (mammalian collagenase and acid cathepsin) activity was assessed biochemically. B. gingivalis was present in oral samples from all monoinfected rats, and no contaminating bacteria were detected in any oral or fecal sample. Animals immunized with B. gingivalis cells had elevated serum and saliva antibodies to whole cells and partially purified fimbriae from B. gingivalis. Infected sham-immunized rats had significantly more periodontal bone loss than noninfected controls, whereas the periodontal bone level in infected rats immunized with 10(10) B. gingivalis cells was similar to that of the noninfected controls. The activities of gingival collagenase and cathepsin B and L were high in sham-immunized infected rats and low in all other animal groups. In conclusion, it is possible to reduce B. gingivalis-induced periodontal tissue loss in gnotobiotic rats by immunization.
牙龈拟杆菌与多种形式的牙周疾病有关。为了评估免疫反应在调节牙龈拟杆菌相关牙周疾病中的作用，在悉生大鼠中评估了牙龈拟杆菌诱导的牙周骨丢失的免疫效果。用不同剂量的全细胞免疫的雄性斯普拉格-道利大鼠或用不完全弗氏佐剂进行假免疫的大鼠，通过灌胃用羧甲基纤维素单感染牙龈拟杆菌。另外两组分别作为假免疫或未处理的无菌对照。感染后42天，处死所有大鼠，通过形态学和放射学方法评估牙周骨水平，并通过生化方法评估牙龈蛋白酶（哺乳动物胶原酶和酸性组织蛋白酶）活性。所有单感染大鼠的口腔样本中均存在牙龈拟杆菌，在任何口腔或粪便样本中均未检测到污染细菌。用牙龈拟杆菌细胞免疫的动物血清和唾液中针对全细胞和从牙龈拟杆菌中部分纯化的菌毛的抗体升高。感染的假免疫大鼠的牙周骨丢失明显多于未感染的对照，而用10(10)个牙龈拟杆菌细胞免疫的感染大鼠的牙周骨水平与未感染对照相似。假免疫感染大鼠的牙龈胶原酶、组织蛋白酶B和L的活性高，而所有其他动物组的活性低。总之，在悉生大鼠中通过免疫有可能减少牙龈拟杆菌诱导的牙周组织丢失。
