Li Wei
Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Analyst. 2006 Jul;131(7):777-81. doi: 10.1039/b605110c.
In vivo analysis in whole cell bacteria, especially the native tertiary structures of the bacterial cell wall, remains an unconquered frontier. The current understanding of bacterial cell wall structures has been based on destructive analysis of individual components. These in vitro results may not faithfully reflect the native structural and conformational information. Multidimensional High Resolution Magic Angle Spinning NMR (HRMAS NMR) has evolved to be a powerful technique in a variety of in vivo studies, including live bacterial cells. Existing studies of HRMAS NMR in bacteria, technical consideration of its successful application, and current limitations in studying true human pathogens are briefly reviewed in this report.
对全细胞细菌进行体内分析，尤其是细菌细胞壁的天然三级结构，仍然是一个未被攻克的领域。目前对细菌细胞壁结构的理解是基于对单个成分的破坏性分析。这些体外研究结果可能无法如实反映天然的结构和构象信息。多维高分辨率魔角旋转核磁共振（HRMAS NMR）已发展成为一种在包括活细菌细胞在内的各种体内研究中强有力的技术。本报告简要回顾了细菌中HRMAS NMR的现有研究、其成功应用的技术考量以及目前在研究真正的人类病原体方面的局限性。
