紫贻贝ABRM中的多种紫贻贝抑制肽：分离与表征

A variety of Mytilus inhibitory peptides in the ABRM of Mytilus edulis: isolation and characterization.

Fujisawa Y, Kubota I, Ikeda T, Minakata H, Muneoka Y

Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1991;100(3):525-31.

Abstract

Five species of Mytilus inhibitory peptides, MIP1-5, were isolated from acetone extracts of the anterior byssus retractor muscle (ABRM) of Mytilus edulis. MIP1 and MIP2 were shown to be S2-MIP and A2-MIP, respectively, first isolated from the pedal ganglia of the animal. 2. All the five peptides had a common C-terminal structure of -Pro-Xaa-Phe-Val-NH2, which was shown to be important for their biological activity. 3. The five MIPs showed similar inhibitory effects on contractions of the ABRM but did not affect catch tension and its relaxation. 4. In addition to the MIPs, catch-relaxing peptide (CARP) was also found in the ABRM.

摘要

从紫贻贝前足丝牵缩肌（ABRM）的丙酮提取物中分离出5种贻贝抑制肽，即MIP1 - 5。MIP1和MIP2分别被证明是S2 - MIP和A2 - MIP，最初是从该动物的足神经节中分离出来的。2. 所有这5种肽都具有共同的C末端结构 -Pro-Xaa-Phe-Val-NH2，这一结构被证明对它们的生物活性很重要。3. 这5种MIP对ABRM的收缩表现出相似的抑制作用，但不影响强直张力及其松弛。4. 除了MIPs之外，在ABRM中还发现了强直松弛肽（CARP）。