Aranda Mario, Morlock Gertrud
Institute of Food Chemistry, University of Hohenheim, Garbenstrasse 28, D-70599 Stuttgart, Germany.
J Chromatogr A. 2006 Oct 27;1131(1-2):253-60. doi: 10.1016/j.chroma.2006.07.018. Epub 2006 Jul 27.
A new high-throughput method was developed to detect simultaneously riboflavin (Vitamin B(2)), pyridoxine (Vitamin B(6)), nicotinamide (Vitamin B(3)), caffeine and taurine in energy drinks by multiple detection. Ten samples of energy drinks and six samples of beverages containing caffeine were prepared by degassing in an ultrasonic bath for 20 min. After chromatography, multi-wavelength scanning was performed by: (1) UV-absorbance measurement at 261 nm for nicotinamide and 275 nm for caffeine, (2) fluorescence measurement at 366/>400 and 313/>340 nm for riboflavin and pyridoxine, respectively, and (3) Vis-absorbance measurement at 525 nm for taurine, after post-chromatographic derivatization with ninhydrin reagent. Calibrations were linear or polynomial with determination coefficients r(2)>0.999. Overall recoveries of the five compounds were between 81 and 106% at three different concentration levels. Repeatabilities (RSD, %) of all substances in matrix were determined to be between 0.8 and 1.5%. Intermediate precisions (RSD, %) ranged between 3.6 and 7.4% for riboflavin, 2.8 and 6.3% for nicotinamide, 2.5 and 4.4% for caffeine, 2.1 and 2.9% for taurine and 0.5 and 4.0% for pyridoxine at different concentration levels. Mass confirmation was performed by a single quadrupole MS in positive electrospray ionisation (ESI) scan mode for all substances except taurine (negative mode). This method offers a good alternative for routine analysis due to its simplicity and at the same time reliability.
开发了一种新的高通量方法，通过多重检测同时测定能量饮料中的核黄素（维生素B2）、吡哆醇（维生素B6）、烟酰胺（维生素B3）、咖啡因和牛磺酸。通过在超声浴中脱气20分钟制备了10个能量饮料样品和6个含咖啡因的饮料样品。色谱分析后，进行多波长扫描：（1）烟酰胺在261nm处和咖啡因在275nm处进行紫外吸光度测量，（2）核黄素和吡哆醇分别在366/>400nm和313/>340nm处进行荧光测量，（3）牛磺酸在茚三酮试剂进行柱后衍生后，在525nm处进行可见吸光度测量。校准为线性或多项式，决定系数r2>0.999。在三个不同浓度水平下，五种化合物的总回收率在81%至106%之间。基质中所有物质的重复性（相对标准偏差，%）测定为0.8%至1.5%。在不同浓度水平下，核黄素的中间精密度（相对标准偏差，%）范围为3.6%至7.4%，烟酰胺为2.8%至6.3%，咖啡因为2.5%至4.4%，牛磺酸为2.1%至2.9%，吡哆醇为0.5%至4.0%。除牛磺酸（负模式）外，所有物质均在正电喷雾电离（ESI）扫描模式下通过单四极杆质谱进行质量确认。该方法因其简单性和可靠性，为常规分析提供了一个很好的选择。
