Balayeva N M, Demkin V V, Rydkina E B, Ignatovich V F, Artemiev M I, Genig V A
N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
Acta Virol. 1993 Dec;37(6):475-83.
A strain of rickettsiae, designated Crimea-108, was isolated from ticks Dermacentor marginatus in the Crimea in 1977. Its immunobiological characteristics involve low pathogenicity for experimental animals, moderate infectivity for chick embryos, and antigenic relatedness to spotted fever group (SFG) rickettsiae (R. sibirica, R. conorii, R. akari), especially to R. sibirica. The genotypic characterization of the strain Crimea-108 was carried out in comparison with SFG and typhus group rickettsiae by using restriction fragment length polymorphism (RFLP) analysis and DNA-probe hybridization. The marked similarity was detected between DNA restriction patterns of the strains Crimea-108, R. sibirica and R. conorii, but each of them besides comigrating fragments had specific ones. Genotypic analysis of the strain Crimea-108, the SFG and typhus group rickettsiae by three independent DNA probes, based on R. prowazekii DNA, gave unique hybridization patterns for the Crimea-108 strain with all probes. The obtained data show that the Crimea-108 isolate does not belong to the species of R. sibirica, R. conorii, R. akari. The strain Crimea-108 is a novel strain of SFG rickettsiae for the Crimea region.
1977年，从克里米亚的边缘革蜱中分离出了一株立克次氏体，命名为克里米亚-108。其免疫生物学特性包括对实验动物致病性低、对鸡胚感染性中等，以及与斑点热群（SFG）立克次氏体（西伯利亚立克次氏体、康氏立克次氏体、小蛛立克次氏体）尤其是与西伯利亚立克次氏体存在抗原相关性。通过限制性片段长度多态性（RFLP）分析和DNA探针杂交，对克里米亚-108菌株与SFG和斑疹伤寒群立克次氏体进行了基因分型鉴定。在克里米亚-108菌株、西伯利亚立克次氏体和康氏立克次氏体的DNA限制性图谱之间检测到明显的相似性，但除了共迁移片段外，它们各自都有特定的片段。基于普氏立克次氏体DNA的三种独立DNA探针，对克里米亚-108菌株、SFG和斑疹伤寒群立克次氏体进行基因分型分析，结果显示克里米亚-108菌株与所有探针都呈现出独特的杂交模式。所获得的数据表明，克里米亚-108分离株不属于西伯利亚立克次氏体、康氏立克次氏体、小蛛立克次氏体的物种。克里米亚-108菌株是克里米亚地区一种新型的SFG立克次氏体菌株。
