Grief C, Farrar G H, Kent K A, Berger E G
National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK.
AIDS. 1991 Dec;5(12):1433-9. doi: 10.1097/00002030-199112000-00004.
Chronic infection of the T-lymphocyte cell line JM with HIV-1 isolate GB8 results in the formation of multinucleate cells (syncytia). Transmission electron microscopy of these syncytia showed the presence of HIV particles both at the cell surface and within cytoplasmic vesicles. HIV particles were observed in dilated Golgi cisternae and Golgi-derived vesicles and in large vacuoles near the periphery of the syncytia. Immunolabelling was performed using an affinity-purified antiserum to the Golgi enzyme galactosyltransferase. This enzyme was consistently localized within both the Golgi apparatus and within virus-containing vesicles of JM syncytia, indicating that these vesicles originated from the Golgi apparatus.
用HIV-1分离株GB8对T淋巴细胞系JM进行慢性感染会导致多核细胞（合胞体）的形成。对这些合胞体进行透射电子显微镜观察显示，在细胞表面和细胞质小泡内均存在HIV颗粒。在扩张的高尔基池、源自高尔基体的小泡以及合胞体外围附近的大液泡中都观察到了HIV颗粒。使用针对高尔基体酶半乳糖基转移酶的亲和纯化抗血清进行免疫标记。该酶始终定位于JM合胞体的高尔基体和含病毒小泡内，表明这些小泡起源于高尔基体。
