Kiernan R, Marshall J, Bowers R, Doherty R, McPhee D
NHMRC Special Unit for AIDS Virology, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia.
AIDS Res Hum Retroviruses. 1990 Jun;6(6):743-52. doi: 10.1089/aid.1990.6.743.
The replication kinetics of HIV were examined in HTLV-I-transformed MT-2 cells. The duration of the initial replication cycle was 20 hours, determined by the first detection of infectious progeny virus, development of syncytia, and production of viral RNA and protein. A phase of exponential virus production followed until 62 h postinfection. Cell death occurred in the final phase of infection during which infectious virus production remained constant even though viral RNA and protein production increased at an exponential rate. Accumulations of HIV particles were observed within cytoplasmic vacuoles of infected MT-2 cells. Although cell lysates contained high titers of infectious virus, our data show that an increasing proportion of particles produced late in infection were not infectious.
在HTLV-I转化的MT-2细胞中检测了HIV的复制动力学。初始复制周期的持续时间为20小时，这是通过首次检测到感染性子代病毒、多核巨细胞的形成以及病毒RNA和蛋白质的产生来确定的。随后是一个病毒指数产生期，直至感染后62小时。细胞死亡发生在感染的最后阶段，在此期间，即使病毒RNA和蛋白质的产生呈指数速率增加，感染性病毒的产生仍保持恒定。在感染的MT-2细胞的细胞质空泡内观察到HIV颗粒的积累。尽管细胞裂解物含有高滴度的感染性病毒，但我们的数据表明，感染后期产生的颗粒中无感染性的比例在增加。
