钙蛋白酶1和钙蛋白酶抑制蛋白对泽西×利木赞、安格斯和赫里福德杂交牛背最长肌熟牛排嫩度的基因型效应。

Genotypic effects of calpain 1 and calpastatin on the tenderness of cooked M. longissimus dorsi steaks from Jersey x Limousin, Angus and Hereford-cross cattle.

作者信息

Morris C A, Cullen N G, Hickey S M, Dobbie P M, Veenvliet B A, Manley T R, Pitchford W S, Kruk Z A, Bottema C D K, Wilson T

机构信息

AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand.

出版信息

Anim Genet. 2006 Aug;37(4):411-4. doi: 10.1111/j.1365-2052.2006.01483.x.

DOI:10.1111/j.1365-2052.2006.01483.x

PMID:16879360

Abstract

Single nucleotide polymorphisms (SNPs) in the calpain 1 (CAPN1) and calpastatin (CAST) genes were studied to determine their effects on meat tenderness in Bos taurus cattle. Strip loins (M. longissimus dorsi) were removed from cattle in four resource populations after slaughter (n = 1042), aged under controlled conditions until fixed times after rigor mortis, cooked and measured using a tenderometer. Animals were genotyped for the CAPN1 SNP c.947C>G (p.Ala316Gly; AF252504) and for the CAST SNP c.2959A>G (AF159246). Frequencies of CAPN1 C alleles ranged from 23% to 68%, and CAST A alleles from 84% to 99.5%. From all data combined, the CAPN1 CC genotype (compared with the GG genotype) was associated with a 20.1 +/- 1.7% reduced average shear force at intermediate stages of ageing (P < 0.001) and with a 9.5 +/- 1.3% reduction near ultimate tenderness (P < 0.001). The heterozygote was intermediate. For CAST, corresponding values for AA compared with AG genotypes were reductions of 8.6 +/- 2.0% and 5.1 +/- 1.6% respectively (both P < 0.001), but there were too few GG genotypes for comparison. There were small interactions between the CAPN1 and CAST genotypes. For the CAPN1 and CAST genotypes combined, the maximal genotype effect in average shear force was 25.7 +/- 5.5% (P < 0.001) at intermediate stages and 15.2 +/- 4.8% near ultimate tenderness (P < 0.01).

摘要

对钙蛋白酶1（CAPN1）基因和钙蛋白酶抑制蛋白（CAST）基因中的单核苷酸多态性（SNP）进行了研究，以确定它们对黄牛牛肉嫩度的影响。屠宰后从四个资源群体的牛身上取下腰大肌（背最长肌）（n = 1042），在受控条件下熟化至尸僵后固定时间，煮熟并用嫩度计测量。对动物进行CAPN1 SNP c.947C>G（p.Ala316Gly；AF252504）和CAST SNP c.2959A>G（AF159246）基因分型。CAPN1 C等位基因频率范围为23%至68%，CAST A等位基因频率范围为84%至99.5%。综合所有数据，CAPN1 CC基因型（与GG基因型相比）在熟化中期平均剪切力降低了20.1±1.7%（P < 0.001），在接近最终嫩度时降低了9.5±1.3%（P < 0.001）。杂合子处于中间水平。对于CAST，AA基因型与AG基因型相比，相应的值分别降低了8.6±2.0%和5.1±1.6%（均P < 0.001），但GG基因型数量太少无法进行比较。CAPN1和CAST基因型之间存在微小的相互作用。对于联合的CAPN1和CAST基因型，在熟化中期平均剪切力的最大基因型效应为25.7±5.5%（P < 0.001），在接近最终嫩度时为15.2±4.8%（P < 0.01）。