YidC介导的F1F0 ATP合酶c亚基组装突变体的膜插入。
YidC-mediated membrane insertion of assembly mutants of subunit c of the F1F0 ATPase.
作者信息
Kol Stefan, Turrell Bryony R, de Keyzer Jeanine, van der Laan Martin, Nouwen Nico, Driessen Arnold J M
机构信息
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Materials Science Center Plus, University of Groningen, 9751 NN Haren, The Netherlands.
出版信息
J Biol Chem. 2006 Oct 6;281(40):29762-8. doi: 10.1074/jbc.M605317200. Epub 2006 Jul 31.
YidC is a member of the OxaI family of membrane proteins that has been implicated in the membrane insertion of inner membrane proteins in Escherichia coli. We have recently demonstrated that proteoliposomes containing only YidC support both the stable membrane insertion and the oligomerization of the c subunit of the F(1)F(0) ATP synthase (F(0)c). Here we have shown that two mutants of F(0)c unable to form a functional F(1)F(0) ATPase interact with YidC, require YidC for membrane insertion, but fail to oligomerize. These data show that oligomerization is not essential for the stable YidC-dependent membrane insertion of F(0)c consistent with a function of YidC as a membrane protein insertase.
YidC是膜蛋白OxaI家族的成员，与大肠杆菌内膜蛋白的膜插入有关。我们最近证明，仅含有YidC的蛋白脂质体既支持F(1)F(0) ATP合酶（F(0)c）c亚基的稳定膜插入，也支持其寡聚化。在此我们表明，两个无法形成功能性F(1)F(0) ATP酶的F(0)c突变体与YidC相互作用，膜插入需要YidC，但无法寡聚化。这些数据表明，寡聚化对于F(0)c依赖YidC的稳定膜插入并非必不可少，这与YidC作为膜蛋白插入酶的功能一致。
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