Price Claire E, Driessen Arnold J M
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, NL-9751 NN Haren, The Netherlands.
J Biol Chem. 2008 Oct 3;283(40):26921-7. doi: 10.1074/jbc.M804490200. Epub 2008 Jul 17.
YidC of Escherichia coli belongs to the evolutionarily conserved Oxa1/Alb3/YidC family. Members of this family have all been implicated in membrane protein biogenesis of aerobic respiratory and energy-transducing proteins. YidC is essential for the insertion of subunit c of the F(1)F(0)-ATP synthase and subunit a of cytochrome o oxidase. The aim of this study was to investigate whether YidC plays a role during anaerobic growth of Escherichia coli, specifically when either nitrate or fumarate are used as terminal electron acceptors or under fermentative conditions. The effect of YidC depletion on the growth, enzyme activities, and protein levels in the inner membrane was determined. YidC is essential for all anaerobic growth conditions tested, and this is not because of the decreased levels of F(1)F(0)-ATP synthase in the inner membrane only. The results suggest a role for YidC in the membrane biogenesis of integral membrane parts of the anaerobic respiratory chain.
大肠杆菌的YidC属于进化上保守的Oxa1/Alb3/YidC家族。该家族成员均与需氧呼吸和能量转换蛋白的膜蛋白生物合成有关。YidC对于F(1)F(0)-ATP合酶的c亚基和细胞色素o氧化酶的a亚基的插入至关重要。本研究的目的是调查YidC在大肠杆菌厌氧生长过程中是否发挥作用，特别是当硝酸盐或富马酸盐用作末端电子受体时或在发酵条件下。确定了YidC缺失对内膜生长、酶活性和蛋白质水平的影响。YidC对于所有测试的厌氧生长条件都是必需的，这并非仅仅是因为内膜中F(1)F(0)-ATP合酶水平的降低。结果表明YidC在厌氧呼吸链的整合膜部分的膜生物合成中发挥作用。
