Röling W F M, Ortega-Lucach S, Larter S R, Head I M
School of Civil Engineering and Geosciences and Institute for Research on the Environment and Sustainability, University of Newcastle, Newcastle upon Tyne, United Kingdom.
J Appl Microbiol. 2006 Aug;101(2):290-9. doi: 10.1111/j.1365-2672.2006.02926.x.
Characterization of microbial communities present in a surface petroleum seep in which hydrocarbons have been biodegraded for thousands of years in order to improve the understanding on natural petroleum biodegradation. DNA was extracted from a natural, surface petroleum seep and subjected to culture independent analysis (rRNA gene-based denaturing gradient gel electrophoresis and phylogenetic analysis of clone libraries). Molecular analysis suggested dominance by acidophilic bacteria, especially Alphaproteobacteria (mainly bacteria related to Acidiphilium and Acidocella). Archaea were not detected, but fungi were present. pH of the samples was around 3.5. Acidophilic microbial communities are associated with an acidic petroleum seep. Microbial community structure analysis gives information on the environmental conditions under which petroleum biodegradation occurs. This knowledge could be applied to define conditions for specific cultivation or activity measurements. The activity of acidophilic micro-organisms deserves more attention with respect to their involvement in natural petroleum degradation. This knowledge will contribute to the design of oil bioremediation strategies for polluted acidic settings.
对一个表层石油渗漏点中存在的微生物群落进行表征，该渗漏点中的碳氢化合物已经经历了数千年的生物降解，目的是增进对天然石油生物降解的理解。从一个天然的表层石油渗漏点提取DNA，并进行非培养分析（基于rRNA基因的变性梯度凝胶电泳和克隆文库的系统发育分析）。分子分析表明嗜酸细菌占主导地位，尤其是α-变形菌纲（主要是与嗜酸菌属和酸单胞菌属相关的细菌）。未检测到古菌，但存在真菌。样品的pH值约为3.5。嗜酸微生物群落与酸性石油渗漏有关。微生物群落结构分析提供了有关石油生物降解发生的环境条件的信息。这些知识可用于确定特定培养或活性测量的条件。嗜酸微生物在天然石油降解中的作用值得更多关注。这些知识将有助于设计受污染酸性环境的石油生物修复策略。
