Li Li, Li Cheng-Tao, Li Rong-Yu, Liu Yan, Lin Yuan, Que Ting-Zhi, Sun Mei-Qian, Li Yao
Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, PR China.
Forensic Sci Int. 2006 Oct 16;162(1-3):74-9. doi: 10.1016/j.forsciint.2006.06.010. Epub 2006 Aug 1.
Research on the application feasibility of SNP genotyping for forensic identification by microarrays.
Oligonucleotide microarrays which could detect 34 different SNPs were used. After hybridization and washing, the arrays were scanned and fluorescence intensities analyzed using Microarray software. Population studies on 34 SNP loci were carried out in a sample of 109 unrelated Chinese Han individuals using oligonucleotide microarrays for genotype detection. The method was also applied to cases.
According to the results of population studies, no deviations from Hardy-Weinberg equilibrium could be found. Among the 34 loci, 3 SNPs were low informative, 4 were medium informative and 27 were high informative. The combination discrimination power (CDP) of the 31 optimal polymorphic SNPs was 0.9999999999979. The matching probability was 2.13 x 10(-12). The average exclusion probability in paternity testing for duos was 0.9609. The average exclusion probability in paternity testing for trios was 0.9970.
The data and case application demonstrated that SNP typing by oligonucleotide probe microarrays was a useful technique for paternity testing and individual identification. Combined with the 28 SNPs loci distributed on HLA-DRB1 and ABO genes, the combination discrimination power (CDP) was 0.9999999999999910. The matching probability was 9.02 x 10(-15). The average exclusion probabilities in duos and in trios were 0.9894 and 0.9992, respectively. It may be concluded that the 59 SNPs loci yield the same power in forensic identification as CODIS STRs currently used.
研究微阵列SNP基因分型用于法医鉴定的应用可行性。
使用能够检测34种不同SNP的寡核苷酸微阵列。杂交和洗涤后，扫描阵列并使用微阵列软件分析荧光强度。采用寡核苷酸微阵列进行基因分型检测，对109名无亲缘关系的中国汉族个体样本进行了34个SNP位点的群体研究。该方法也应用于案例分析。
根据群体研究结果，未发现偏离哈迪-温伯格平衡的情况。在34个位点中，3个SNP信息性低，4个信息性中等，27个信息性高。31个最佳多态性SNP的组合鉴别力（CDP）为0.9999999999979。匹配概率为2.13×10⁻¹²。二联体亲子鉴定的平均排除概率为0.9609。三联体亲子鉴定的平均排除概率为0.9970。
数据和案例应用表明，寡核苷酸探针微阵列SNP分型是亲子鉴定和个体识别的有用技术。与分布在HLA-DRB1和ABO基因上的28个SNP位点相结合，组合鉴别力（CDP）为0.9999999999999910。匹配概率为9.02×10⁻¹⁵。二联体和三联体的平均排除概率分别为0.9894和0.9992。可以得出结论，59个SNP位点在法医鉴定中的效力与目前使用的CODIS STRs相同。
