Imboden J B, June C H, McCutcheon M A, Ledbetter J A
Department of Medicine, University of California, San Francisco.
J Clin Invest. 1990 Jan;85(1):130-4. doi: 10.1172/JCI114402.
After the addition of a CD3 monoclonal antibody to peripheral T cells that have been previously stimulated with phytohemagglutinin, inositol phosphates are produced at a rapid rate for 2 min and at a much slower rate thereafter. Stimulation of CD5 allows CD3-mediated production of inositol phosphates to be sustained at a brisk rate for greater than 20 min and augments the initial CD3-mediated increase in inositol trisphosphate and release of intracellular Ca2+. Thus, perturbation of CD5 by monoclonal antibody enhances the ability of the CD3-antigen receptor complex to couple to the inositol phospholipid pathway. This effect of CD5 is independent of any direct effect of the CD5 monoclonal antibody on the levels of inositol phosphates.
在向先前已用植物血凝素刺激过的外周T细胞中添加CD3单克隆抗体后，肌醇磷酸在2分钟内快速产生，之后速率则慢得多。CD5的刺激可使CD3介导的肌醇磷酸产生以较快速率持续超过20分钟，并增强最初CD3介导的肌醇三磷酸增加和细胞内Ca2+的释放。因此，单克隆抗体对CD5的干扰增强了CD3抗原受体复合物与肌醇磷脂途径偶联的能力。CD5的这种作用与CD5单克隆抗体对肌醇磷酸水平的任何直接作用无关。
