Wybo I, Van Blerk M, Malfait R, Goubert P, Gorus F
Department of Clinical Chemistry, Academic Hospital, Vrije Universiteit Brussel, Belgium.
Clin Chem. 1990 Jan;36(1):123-5.
Pharmacia's "PhastSystem" for semi-automated isoelectric focusing (IEF) in thin precast polyacrylamide gels (PAGE) was found to be as sensitive as high-resolution protein electrophoresis (HRPE) in agarose gels and conventional PAGE-IEF for detection of oligoclonal banding (OB) in concentrated cerebrospinal fluid (CSF) samples. Both PhastSystem IEF and HRPE revealed OB in CSF from eight of nine multiple sclerosis patients and four of 10 patients with various types of infection of the central nervous system as opposed to only two of 70 patients with miscellaneous neuropsychiatric disorders. The PhastSystem also frequently detected OB in silver-stained, unconcentrated CSF from patients with multiple sclerosis.
在检测浓缩脑脊液（CSF）样本中的寡克隆区带（OB）时，发现Pharmacia公司用于在预制薄聚丙烯酰胺凝胶（PAGE）中进行半自动等电聚焦（IEF）的“PhastSystem”，与琼脂糖凝胶中的高分辨率蛋白质电泳（HRPE）以及传统的PAGE-IEF一样灵敏。在九名多发性硬化症患者的脑脊液样本中，PhastSystem IEF和HRPE均在其中八名患者中检测到OB；在10名患有各种类型中枢神经系统感染的患者中，有四名检测到OB；而在70名患有各种神经精神疾病的患者中，只有两名检测到OB。PhastSystem还经常在多发性硬化症患者经银染的未浓缩脑脊液中检测到OB。
