亲和免疫印迹法：通过琼脂糖等电聚焦快速灵敏地检测未浓缩脑脊液中的寡克隆IgG、IgA和IgM。

Affinity immunoblotting: rapid and sensitive detection of oligoclonal IgG, IgA and IgM in unconcentrated CSF by agarose isoelectric focusing.

作者信息

Kaiser R

机构信息

Department of Neurology, University of Freiburg, Germany.

出版信息

J Neurol Sci. 1991 Jun;103(2):216-25. doi: 10.1016/0022-510x(91)90167-6.

DOI:10.1016/0022-510x(91)90167-6

Abstract

Two methods for the investigation of oligoclonal immunoglobulin bands in cerebrospinal fluid are compared. Isoelectric focusing (IEF) in agarose gels combined with a highly sensitive affinity immunoblotting proved to be superior to PAGE-IEF and silver staining regarding detection sensitivity and expenditure of labour. The procedure presented here allows examination of oligoclonal bands not only of IgG, but also of IgA and IgM antibodies in less than 4 h. Oligoclonal IgM bands could be detected in 6 patients with neuroborreliosis or neurosyphilis as well as in one patient with a peripheral neuropathy where other electrophoretic methods, including immunoelectrophoresis, were negative. Oligoclonal IgA bands were demonstrated only in 2 patients with a bacterial infection of the CNS. In patients with multiple sclerosis amounts of IgM and IgA in the CSF were too low to allow investigation of oligoclonal IgM or IgA bands.

摘要

比较了两种检测脑脊液中寡克隆免疫球蛋白带的方法。琼脂糖凝胶等电聚焦（IEF）结合高度灵敏的亲和免疫印迹法在检测灵敏度和劳动强度方面优于聚丙烯酰胺凝胶等电聚焦（PAGE-IEF）和银染法。本文介绍的方法能够在不到4小时的时间内不仅检测IgG的寡克隆带，还能检测IgA和IgM抗体的寡克隆带。在6例神经莱姆病或神经梅毒患者以及1例其他电泳方法（包括免疫电泳）均为阴性的周围神经病患者中检测到了寡克隆IgM带。仅在2例中枢神经系统细菌感染患者中检测到了寡克隆IgA带。在多发性硬化症患者中，脑脊液中IgM和IgA的含量过低，无法检测寡克隆IgM或IgA带。