缺乏细胞色素的大肠杆菌中膜结合呼吸硝酸盐还原酶（EC1.7.99.4）的合成及不对称性
Synthesis and sideedness of membrane-bound respiratory nitrate reductase (EC1.7.99.4) in Escherichia coli lacking cytochromes.
出版信息
Biochem J. 1975 May;148(2):329-33.
Abstract
The synthesis of nitrate reductase and its incorporation into the cytoplasmic membrane of Escherichia coli strain A1004a (5-aminolaevulinic acid auxotroph) does not require synthesis of cytochrome b. The synthesis of the apoprotein(s) of the cytochrome b of the respiratory pathway from NADH to nitrate appears to be inhibited by the absence of haem. No member of the respiratory pathway from NADH to oxygen is capable of reducing nitrate reductase directly. The site on nitrate reductase that oxidizes FMNH2 is located on the cytoplasmic aspect of the cytoplasmic membrane.
摘要
大肠杆菌菌株A1004a（5-氨基乙酰丙酸营养缺陷型）中硝酸还原酶的合成及其整合到细胞质膜中并不需要细胞色素b的合成。从NADH到硝酸盐的呼吸途径中细胞色素b的脱辅基蛋白的合成似乎因血红素的缺失而受到抑制。从NADH到氧气的呼吸途径中没有成员能够直接还原硝酸还原酶。硝酸还原酶上氧化FMNH2的位点位于细胞质膜的细胞质一侧。
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