Suenaga T, Hirano A, Llena J F, Ksiezak-Reding H, Yen S H, Dickson D W
Department of Pathology, Montefiore Medical Center, Bronx, NY 10467.
J Neuropathol Exp Neurol. 1990 Jan;49(1):31-40. doi: 10.1097/00005072-199001000-00004.
Senile plaques (SP) in the cerebellum of 23 cases of Alzheimer's disease (AD), three with widespread amyloid angiopathy, were studied with a modified Bielschowsky stain and immunocytochemical methods using antibodies to a beta-amyloid synthetic peptide (beta ASP), phosphorylated neurofilament proteins, ubiquitin, tau protein, and glial fibrillary acidic protein (GFAP). The four subtypes of SP (diffuse plaques, compact plaques, perivascular plaques, and subpial fibrillar deposits) that were observed with the modified Bielschowsky stain were also stained with antibodies to beta ASP. Many cerebellar SP contained ubiquitin-positive granular elements resembling dystrophic neurites. In contrast to neuritic elements in cerebral SP in AD, ubiquitin-positive elements in cerebellar SP were not labeled with antibodies to phosphorylated neurofilament or tau proteins. Various degrees of glial reaction were observed in all subtypes of SP except diffuse plaques. The absence of phosphorylated neurofilament and tau epitopes in neuritic elements in cerebellar SP is not surprising since paired helical filaments have not been seen in the cerebellum. Nevertheless, our results suggest that cerebellar SP are frequently associated with dystrophic neurites.
对23例阿尔茨海默病（AD）患者小脑的老年斑（SP）进行了研究，其中3例伴有广泛的淀粉样血管病，采用改良的 Bielschowsky 染色和免疫细胞化学方法，使用针对β淀粉样合成肽（βASP）、磷酸化神经丝蛋白、泛素、tau蛋白和胶质纤维酸性蛋白（GFAP）的抗体。用改良的 Bielschowsky 染色观察到的SP的四种亚型（弥漫性斑块、致密性斑块、血管周围斑块和软脑膜下纤维状沉积物）也用βASP抗体染色。许多小脑SP含有类似于营养不良性神经突的泛素阳性颗粒成分。与AD脑SP中的神经突成分不同，小脑SP中的泛素阳性成分未被磷酸化神经丝或tau蛋白抗体标记。除弥漫性斑块外，在所有SP亚型中均观察到不同程度的胶质反应。小脑SP神经突成分中缺乏磷酸化神经丝和tau表位并不奇怪，因为在小脑中未发现双螺旋丝。然而，我们的结果表明，小脑SP经常与营养不良性神经突相关。
