Nowotschin Sonja, Hadjantonakis Anna-Katerina
Developmental Biology Program, Sloan-Kettering Institute, New York, NY, USA.
Methods Mol Biol. 2014;1092:405-20. doi: 10.1007/978-1-60327-292-6_24.
The use of genetically encoded fluorescent proteins has revolutionized the fields of cell and developmental biology and redefined our understanding of the dynamic morphogenetic processes that work to shape the embryo. Fluorescent proteins are routinely used as vital reporters to label tissues, cells, cellular organelles, or proteins of interest and in doing so provide contrasting agents enabling the acquisition of high-resolution quantitative image data. With the advent of more accessible and sophisticated imaging technologies and abundance of fluorescent proteins with different spectral characteristics, the dynamic processes taking place in situ in living embryos can now be probed. Here, we provide an overview of some recent advances in this rapidly evolving field.
基因编码荧光蛋白的应用彻底改变了细胞生物学和发育生物学领域，并重新定义了我们对塑造胚胎的动态形态发生过程的理解。荧光蛋白通常用作重要的报告分子，用于标记组织、细胞、细胞器或感兴趣的蛋白质，从而提供对比剂，使我们能够获取高分辨率的定量图像数据。随着更易于使用和更先进的成像技术的出现，以及具有不同光谱特征的荧光蛋白的大量涌现，现在可以对活胚胎原位发生的动态过程进行探究。在此，我们概述了这个快速发展领域的一些最新进展。
