Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Curr Opin Cell Biol. 2011 Jun;23(3):310-7. doi: 10.1016/j.ceb.2010.12.004. Epub 2011 Jan 15.
Recent developments in reagent design can address problems in single cells that were not previously approachable. We have attempted to foresee what will become possible, and the sorts of biological problems that become tractable with these novel reagents. We have focused on the novel fluorescent proteins that allow convenient multiplexing, and provide for a time-dependent analysis of events in single cells. Methods for fluorescently labeling specific molecules, including endogenously expressed proteins and mRNA have progressed and are now commonly used in a variety of organisms. Finally, sensitive microscopic methods have become more routine practice. This article emphasizes that the time is right to coordinate these approaches for a new initiative on single cell imaging of biological molecules.
近年来，试剂设计方面的进展可以解决以前无法解决的单细胞问题。我们试图预见未来可能实现的情况，以及利用这些新型试剂解决的生物学问题类型。我们专注于新型荧光蛋白，它们可以方便地进行多重标记，并提供对单细胞中事件的时间依赖性分析。荧光标记特定分子的方法，包括内源性表达的蛋白质和 mRNA，已经取得进展，现在在各种生物体中都得到了广泛应用。最后，灵敏的显微镜方法已经成为更常规的做法。本文强调，现在是协调这些方法以开展生物分子单细胞成像新计划的好时机。
