[瓦斯爆炸对大鼠肺组织中核因子-κB和细胞间黏附分子-1表达的影响及意义]
[Effects and significance of gas explosion on expression of NF-kappaB and ICAM1 in lung tissue of rats].
作者信息
Liu Lin-hong, Ren Wen-jie, Wang Gui-zhen, Qin Zu-xuan, Wang Xiao-min, Wu Song-yuan, Liu Ya, Liu Liang-ming
机构信息
General Hospital of Coal Mining Group of Pingdingshan, Henan Province 467000, China.
出版信息
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Jul;24(7):409-11.
OBJECTIVE
To explore the expression of NF-kappaB and ICAM-1 in the gas explosion wounded lung of rats and the relationship.
METHODS
Digoxin labeled NF-kappaB was used as probe. In situ hybridization was performed to detect the NF-kappaB mRNA. Immunohistochemistry was used to detect the expression of NF-kappaB and ICAM-1.
RESULTS
The levels of NF-kappaB mRNA, the expression of NF-kappaB and ICAM-1 in the wounded rats were significantly increased and reached their peak two hours after injury. Pathology of lung tissue showed that some crachea epithelium mucosae were desquamated; congestion, edema of trachea wall and infiltration of neutrophilic granulocytes were found; hemorrhage, edema and infiltration of lots of inflammatory cells were present in alveolus cells. Electron microscope showed that type I, especially type II alveolus epithelia had degeneration and desquamation.
CONCLUSION
The injury of gas explosion can activate NF-kappaB, which has close correlation with the acute injury to lung.
目的
探讨核因子κB（NF-κB）和细胞间黏附分子-1（ICAM-1）在大鼠气体爆炸伤肺组织中的表达及二者关系。
方法
用地高辛标记的NF-κB作为探针，采用原位杂交法检测NF-κB mRNA，用免疫组织化学法检测NF-κB和ICAM-1的表达。
结果
伤后大鼠肺组织中NF-κB mRNA水平、NF-κB和ICAM-1表达均显著升高，并于伤后2小时达高峰。肺组织病理学检查显示部分气管黏膜上皮脱落，气管壁充血、水肿，有中性粒细胞浸润；肺泡腔内有出血、水肿及大量炎性细胞浸润。电镜检查显示Ⅰ型尤其是Ⅱ型肺泡上皮细胞变性、脱落。
结论
气体爆炸伤可激活NF-κB，其与肺组织急性损伤密切相关。
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