Plant Cell Biotechnology Department, Central Food Technological Research Institute, Cheluvamba Mansion, Mysore 570 020, Karnataka, India.
Hepatol Res. 2005 Dec;33(4):313-9. doi: 10.1016/j.hepres.2005.08.008.
This is the first report on the hepatoprotective potentials of marine micro algae Dunaliella species. Dunaliella salina, halotolarent green alga was cultivated in modified autotrophic medium. The alga was subjected to light and nutrient stress in order to accumulate (beta-carotene along with other carotenoids. Such beta-carotene enriched yellow cells were fed to rats by mixing with regular feed at the dose of 2.5 and of 5.0gkg(-1) b.w. for 2 weeks. The degree of hepatoprotection was measured up on challenging animals with toxin (2.0gkg(-1) of carbon tetrachloride) by estimation of biochemical parameters like, serum transaminases (serum aspartate transaminase (S)AST and serum alanine transaminase (S)ALT), serum alkaline phosphatase and total protein. The results were compared to animals on normal diet and with group fed with 100mugkg(-1) b.w. of standard all trans beta-carotene. Among the three test groups the group fed with algae of 5.0gkg(-1) body weight, showed maximum protection. The levels of (S)AST and (S)ALT was found to be 61.3+/-6.4 and 80.7+/-5.6%, against 90.8+/-10.5 and 144.7+/-13.9% in case of standard beta-carotene. The protein contents were increased in case of control to 6.1+/-0.7 and the same was found to be significantly less in case of 5.0gkg(-1)Dunaliella fed group, which shown 5.6+/-0.8% total protein. However, the activity of 2.5gkg(-1) was also significant comparatively (P<0.05). The results indicate that Dunaliella, which contains isomeric forms of beta-carotene can act as good antihepatotoxic when compared to synthetic all trans beta-carotene. Dunaliella has shown the presence of both cis and trans isomeric forms of beta-carotene, where as synthetic compounds contain only trans isomer. Hepatoprotectivity may be due to presence of various isomeric forms of carotene and other oxygenated carotenoids (xanthophylls) in algae.
这是第一篇关于海洋微藻杜氏盐藻（Dunaliella salina）的保肝潜力的报告。杜氏盐藻是一种耐盐的绿色藻类，在改良的自养培养基中进行培养。为了积累（β-胡萝卜素和其他类胡萝卜素，使藻类受到光和营养胁迫。将富含β-胡萝卜素的黄色细胞与常规饲料混合，以 2.5 和 5.0gkg(-1) 的剂量喂养大鼠，为期 2 周。通过估计生化参数（血清天冬氨酸转氨酶（S）AST 和血清丙氨酸转氨酶（S）ALT）、血清碱性磷酸酶和总蛋白，来衡量毒素（2.0gkg(-1)的四氯化碳）对动物的保护程度。将这些结果与正常饮食的动物和 100μgkg(-1) 体重的标准全反式β-胡萝卜素喂养的动物进行比较。在三个实验组中，以 5.0gkg(-1) 体重的藻类喂养的组显示出最大的保护作用。发现 S）AST 和 S）ALT 的水平分别为 61.3+/-6.4 和 80.7+/-5.6%，而标准β-胡萝卜素组的水平分别为 90.8+/-10.5 和 144.7+/-13.9%。对照组的蛋白质含量增加到 6.1+/-0.7，而以 5.0gkg(-1) 杜氏盐藻喂养的组则发现显著减少，仅为 5.6+/-0.8%的总蛋白。然而，2.5gkg(-1) 的活性也具有统计学意义（P<0.05）。结果表明，杜氏盐藻含有β-胡萝卜素的异构体，与合成的全反式β-胡萝卜素相比，可以作为良好的抗肝毒性物质。杜氏盐藻含有顺式和反式异构体的β-胡萝卜素，而合成化合物仅含有反式异构体。保肝作用可能是由于藻类中存在各种类胡萝卜素异构体和其他含氧类胡萝卜素（叶黄素）。
