Hutt Kenji, Hernandez Randy, Heagy Michael D
New Mexico Institute of Mining and Technology, Chemistry, 801 Leroy Avenue, Socorro, NM 87801, USA.
Bioorg Med Chem Lett. 2006 Oct 15;16(20):5436-8. doi: 10.1016/j.bmcl.2006.07.050. Epub 2006 Aug 4.
A fluorescent probe (1), developed for recognition of alpha helical secondary structure, shows a large fluorescence change upon titration with the synthetic protein PBLG. Compared to fluorophores of similar size and shape, 1 displayed the smallest dissociation constant (K(D)=80microM) when titrated with PBLG. These preliminary studies are directed toward developing small molecule proteomimetics that have intrinsic fluorescence and are specific for helical-protein binding-sites.
