Grzelak Agnieszka, Macierzyńska Ewa, Bartosz Grzegorz
Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
Exp Gerontol. 2006 Sep;41(9):813-8. doi: 10.1016/j.exger.2006.06.049. Epub 2006 Aug 7.
Comparison of senescent yeast obtained by the "baby machine" technique with 2-day-old stationary phase cells revealed decreased activities of glutathione reductase, glutathione S-transferase, glutathione peroxidase and alcohol dehydrogenase, reduction of total antioxidant capacity, protein glycation and accumulation of products of oxidative damage: protein carbonyls and DNA damage assessed by augmented content of 8-oxoguanine and increased tail momentum of cellular DNA in the comet assay. These results are consistent with a role for oxidative damage during replicative senescence of Saccharomyces cerevisiae.
通过“幼芽机”技术获得的衰老酵母与2日龄稳定期细胞的比较显示，谷胱甘肽还原酶、谷胱甘肽S-转移酶、谷胱甘肽过氧化物酶和乙醇脱氢酶的活性降低，总抗氧化能力下降，蛋白质糖基化以及氧化损伤产物积累：蛋白质羰基化和DNA损伤通过彗星试验中8-氧代鸟嘌呤含量增加和细胞DNA尾部动量增加来评估。这些结果与氧化损伤在酿酒酵母复制性衰老过程中的作用一致。
