• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

评估用于检测临床样本中结核分枝杆菌复合群和四种非结核分枝杆菌的GenoType分枝杆菌直接检测法。

Evaluation of the GenoType Mycobacteria Direct assay for detection of Mycobacterium tuberculosis complex and four atypical mycobacterial species in clinical samples.

作者信息

Franco-Alvarez de Luna F, Ruiz P, Gutiérrez J, Casal M

机构信息

Microbiology Service, Reina Sofia University Hospital, and Mycobacteria Reference Center, Faculty of Medicine, Cordoba University, Cordoba, Spain.

出版信息

J Clin Microbiol. 2006 Aug;44(8):3025-7. doi: 10.1128/JCM.00068-06.

DOI:10.1128/JCM.00068-06
PMID:16891533
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1594618/
Abstract

We evaluated GenoType Mycobacteria Direct (GTMD), a novel commercial assay based on nucleic acid sequence-based amplification technology, for the detection of Mycobacterium tuberculosis complex, M. avium, M. intracellulare, M. kansasii, and M. malmoense directly from clinical specimens. A total of 134 respiratory and extrapulmonary samples from 65 patients were processed. Sensitivity, specificity, positive predictive, and negative predictive values for GTMD were 92, 100, 100, and 77%, respectively. The GMTD technique is useful, reliable, and rapid when used during the normal routine of a clinical laboratory.

摘要

我们评估了基于核酸序列扩增技术的新型商业检测方法——基因分型分枝杆菌直接检测法（GTMD），用于直接从临床标本中检测结核分枝杆菌复合群、鸟分枝杆菌、胞内分枝杆菌、堪萨斯分枝杆菌和马尔默分枝杆菌。对65例患者的134份呼吸道和肺外样本进行了检测。GTMD的敏感性、特异性、阳性预测值和阴性预测值分别为92%、100%、100%和77%。在临床实验室的常规检测中使用时，GMTD技术是有用、可靠且快速的。

相似文献

1
Evaluation of the GenoType Mycobacteria Direct assay for detection of Mycobacterium tuberculosis complex and four atypical mycobacterial species in clinical samples.评估用于检测临床样本中结核分枝杆菌复合群和四种非结核分枝杆菌的GenoType分枝杆菌直接检测法。
J Clin Microbiol. 2006 Aug;44(8):3025-7. doi: 10.1128/JCM.00068-06.
2
Evaluation of the GenoType Mycobacteria Direct assay for the simultaneous detection of the Mycobacterium tuberculosis complex and four atypical mycobacterial species in smear-positive respiratory specimens.评估GenoType分枝杆菌直接检测法在涂片阳性呼吸道标本中同时检测结核分枝杆菌复合群和四种非结核分枝杆菌的性能。
J Med Microbiol. 2008 May;57(Pt 5):605-611. doi: 10.1099/jmm.0.47484-0.
3
Evaluation of a membrane array for detection of Mycobacterium tuberculosis complex and nontuberculous mycobacteria in positive liquid cultures.膜阵列检测阳性液体培养物中的结核分枝杆菌复合群和非结核分枝杆菌的评估。
Diagn Microbiol Infect Dis. 2013 Apr;75(4):337-41. doi: 10.1016/j.diagmicrobio.2013.01.009.
4
Evaluation of the GenoType Mycobacteria Direct assay for direct detection of the Mycobacterium tuberculosis complex obtained from sputum samples.评价 GenoType 分枝杆菌直接检测法直接检测痰液样本中结核分枝杆菌复合群的效果。
J Med Microbiol. 2010 Aug;59(Pt 8):930-934. doi: 10.1099/jmm.0.013490-0. Epub 2010 May 6.
5
Paradigm for diagnosing mycobacterial disease: direct detection and differentiation of complex and non-tuberculous mycobacteria in clinical specimens using multiplex real-time PCR.用于诊断分枝杆菌病的模式：使用多重实时 PCR 直接检测和区分临床标本中的复杂和非结核分枝杆菌。
J Clin Pathol. 2018 Sep;71(9):774-780. doi: 10.1136/jclinpath-2017-204945. Epub 2018 Mar 20.
6
New assay to diagnose and differentiate between Mycobacterium tuberculosis complex and nontuberculous mycobacteria.用于诊断和区分结核分枝杆菌复合群与非结核分枝杆菌的新检测方法。
Tuberculosis (Edinb). 2019 Jan;114:17-23. doi: 10.1016/j.tube.2018.10.004. Epub 2018 Oct 9.
7
Diagnostic Performance of the GENEDIA MTB/NTM Detection Kit for Detecting and Nontuberculous Mycobacteria With Sputum Specimens.GENEDIA MTB/NTM 检测试剂盒检测痰液标本中结核分枝杆菌和非结核分枝杆菌的诊断性能。
Ann Lab Med. 2020 Mar;40(2):169-173. doi: 10.3343/alm.2020.40.2.169.
8
Development of a One-Step Multiplex PCR Assay for Differential Detection of Major Mycobacterium Species.一种用于主要分枝杆菌菌种鉴别检测的一步多重PCR检测方法的开发。
J Clin Microbiol. 2017 Sep;55(9):2736-2751. doi: 10.1128/JCM.00549-17. Epub 2017 Jun 28.
9
Molecular detection and identification of mycobacterium tuberculosis complex and four clinically important nontuberculous mycobacterial species in smear-negative clinical samples by the genotype mycobacteria direct test.基因分型分枝杆菌直接检测法检测和鉴定痰涂片阴性临床标本中的结核分枝杆菌复合群和四种重要的临床非结核分枝杆菌。
J Clin Microbiol. 2011 Aug;49(8):2874-8. doi: 10.1128/JCM.00612-11. Epub 2011 Jun 8.
10
Simultaneous detection of Mycobacterium tuberculosis complex and nontuberculous mycobacteria in respiratory specimens.同时检测呼吸道标本中的结核分枝杆菌复合体和非结核分枝杆菌。
Tuberculosis (Edinb). 2013 Nov;93(6):642-6. doi: 10.1016/j.tube.2013.07.007. Epub 2013 Aug 7.

引用本文的文献

1
Development and evaluation of a multiplex molecular point-of-care assay for direct identification of and prioritized non-tuberculous mycobacteria.一种用于直接鉴定和优先排序非结核分枝杆菌的多重分子即时检测方法的开发与评估
Front Cell Infect Microbiol. 2025 Aug 13;15:1560870. doi: 10.3389/fcimb.2025.1560870. eCollection 2025.
2
Prevalence of in clinical and environmental isolates, a systematic review and meta-analysis.临床和环境分离株中……的患病率：一项系统评价和荟萃分析。 （原文中“Prevalence of ”后面缺少具体内容）
Front Microbiol. 2024 Feb 19;15:1321273. doi: 10.3389/fmicb.2024.1321273. eCollection 2024.
3
Performance of Interferon-Gamma Release Assays in the Diagnosis of Nontuberculous Mycobacterial Diseases-A Retrospective Survey From 2011 to 2019.γ-干扰素释放试验在非结核分枝杆菌病诊断中的应用性能：一项 2011 至 2019 年的回顾性调查。
Front Cell Infect Microbiol. 2021 Feb 18;10:571230. doi: 10.3389/fcimb.2020.571230. eCollection 2020.
4
Species Typing of Nontuberculous Mycobacteria by Use of Deoxyribozyme Sensors.脱氧核酶传感器在非结核分枝杆菌种型鉴定中的应用。
Clin Chem. 2019 Feb;65(2):333-341. doi: 10.1373/clinchem.2018.295212. Epub 2018 Dec 6.
5
Molecular Mycobacteriology and Expansion in Disease Diagnosis.分子分枝杆菌学与疾病诊断的拓展
Indian J Clin Biochem. 2016 Apr;31(2):138-47. doi: 10.1007/s12291-015-0504-2. Epub 2015 Apr 28.
6
US Cystic Fibrosis Foundation and European Cystic Fibrosis Society consensus recommendations for the management of non-tuberculous mycobacteria in individuals with cystic fibrosis.美国囊性纤维化基金会和欧洲囊性纤维化协会关于囊性纤维化患者非结核分枝杆菌管理的共识建议。
Thorax. 2016 Jan;71 Suppl 1(Suppl 1):i1-22. doi: 10.1136/thoraxjnl-2015-207360.
7
Incubation time of Mycobacterium tuberculosis complex sputum cultures in BACTEC MGIT 960: 4weeks of negative culture is enough for physicians to consider alternative diagnoses.结核分枝杆菌复合群痰液培养物在BACTEC MGIT 960中的孵育时间：培养4周阴性足以让医生考虑其他诊断。
Diagn Microbiol Infect Dis. 2015 Oct;83(2):162-4. doi: 10.1016/j.diagmicrobio.2015.07.002. Epub 2015 Jul 9.
8
Molecular detection and identification of mycobacterium tuberculosis complex and four clinically important nontuberculous mycobacterial species in smear-negative clinical samples by the genotype mycobacteria direct test.基因分型分枝杆菌直接检测法检测和鉴定痰涂片阴性临床标本中的结核分枝杆菌复合群和四种重要的临床非结核分枝杆菌。
J Clin Microbiol. 2011 Aug;49(8):2874-8. doi: 10.1128/JCM.00612-11. Epub 2011 Jun 8.
9
Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities.资源匮乏国家的结核病实验室诊断：挑战与机遇。
Clin Microbiol Rev. 2011 Apr;24(2):314-50. doi: 10.1128/CMR.00059-10.
10
Evaluation of the hyplex TBC PCR test for detection of Mycobacterium tuberculosis complex in clinical samples.评价 hyplex TBC PCR 检测试验在临床样本中检测结核分枝杆菌复合群的效果。
BMC Microbiol. 2010 Mar 31;10:95. doi: 10.1186/1471-2180-10-95.

本文引用的文献

1
Comparison of the COBAS AMPLICOR MTB and BDProbeTec ET assays for detection of Mycobacterium tuberculosis in respiratory specimens.COBAS AMPLICOR MTB检测法与BDProbeTec ET检测法在呼吸道标本中检测结核分枝杆菌的比较。
J Clin Microbiol. 2005 Jun;43(6):2563-6. doi: 10.1128/JCM.43.6.2563-2566.2005.
2
Evolution of tuberculosis control and prospects for reducing tuberculosis incidence, prevalence, and deaths globally.全球结核病控制的演变以及降低结核病发病率、患病率和死亡率的前景。
JAMA. 2005 Jun 8;293(22):2767-75. doi: 10.1001/jama.293.22.2767.
3
GenoType mycobacterium assay for identification of mycobacterial species isolated from human clinical samples by using liquid medium.使用液体培养基的基因分型分枝杆菌检测法用于鉴定从人类临床样本中分离出的分枝杆菌种类。
J Clin Microbiol. 2002 Aug;40(8):3076-8. doi: 10.1128/JCM.40.8.3076-3078.2002.
4
Characteristics and applications of nucleic acid sequence-based amplification (NASBA).基于核酸序列的扩增技术（NASBA）的特点与应用
Mol Biotechnol. 2002 Feb;20(2):163-79. doi: 10.1385/MB:20:2:163.
5
Update: Nucleic acid amplification tests for tuberculosis.最新消息：结核病的核酸扩增检测
MMWR Morb Mortal Wkly Rep. 2000 Jul 7;49(26):593-4.
6
Diagnostic Standards and Classification of Tuberculosis in Adults and Children. This official statement of the American Thoracic Society and the Centers for Disease Control and Prevention was adopted by the ATS Board of Directors, July 1999. This statement was endorsed by the Council of the Infectious Disease Society of America, September 1999.《成人及儿童结核病诊断标准与分类》。本美国胸科学会和疾病控制与预防中心的官方声明于1999年7月由美国胸科学会董事会通过。本声明于1999年9月得到美国传染病学会理事会的认可。
Am J Respir Crit Care Med. 2000 Apr;161(4 Pt 1):1376-95. doi: 10.1164/ajrccm.161.4.16141.
7
Evaluation of the COBAS AMPLICOR MTB system.COBAS AMPLICOR 结核分枝杆菌检测系统的评估
J Clin Microbiol. 1997 Jun;35(6):1604-5. doi: 10.1128/jcm.35.6.1604-1605.1997.
8
Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory.核酸扩增技术在临床实验室诊断呼吸道感染中的相关性。
Clin Microbiol Rev. 1997 Apr;10(2):242-56. doi: 10.1128/CMR.10.2.242.
9
NASBA isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection.核酸序列依赖性扩增技术（NASBA）是一种等温酶促体外核酸扩增技术，专为HIV-1感染的诊断而优化。
J Virol Methods. 1991 Dec;35(3):273-86. doi: 10.1016/0166-0934(91)90069-c.