Bian Ka, Ke Yan, Kamisaki Yoshinori, Murad Ferid
The Institute of Molecular Medicine, The University of Texas-Houston Medical School, Houston, TX 77030, USA.
J Pharmacol Sci. 2006 Aug;101(4):271-9. doi: 10.1254/jphs.crj06009x. Epub 2006 Aug 5.
The role of nitric oxide (NO) in cellular signaling has become one of the most rapidly growing areas in biology during the past two decades. As a gas and free radical with an unshared electron, nitric oxide participates in various biological processes. The interaction between NO and proteins may be roughly divided into two categories. In many instances, NO mediates its biological effects by activating guanylyl cyclase and elevates intracellular cyclic GMP synthesis from GTP. However, the list of cGMP-independent effects of NO is also growing at a rapid rate. In this review, the importance and relevance of nitrotyrosine formation are stressed. The utilization of intact cell cultures, tissues, and cell-free preparations along with the use of pharmacological, biochemical, and molecular biological approaches to characterize, purify, and reconstitute these NO regulatory pathways could lead to the development of new therapies for various pathological conditions that are characterized by unbalanced production of NO.
在过去二十年中，一氧化氮（NO）在细胞信号传导中的作用已成为生物学领域发展最为迅速的领域之一。作为一种具有未成对电子的气体和自由基，一氧化氮参与各种生物过程。NO与蛋白质之间的相互作用大致可分为两类。在许多情况下，NO通过激活鸟苷酸环化酶介导其生物学效应，并提高细胞内由GTP合成的环鸟苷酸水平。然而，NO不依赖cGMP的效应列表也在迅速增加。在本综述中，强调了硝基酪氨酸形成的重要性和相关性。利用完整细胞培养物、组织和无细胞制剂，以及使用药理学、生物化学和分子生物学方法来表征、纯化和重建这些NO调节途径，可能会开发出针对各种以NO产生失衡为特征的病理状况的新疗法。
