Long Shao-Hua, Yu Zhu-Qin, Shuai Li, Guo Yun-Liang, Duan De-Lin, Xu Xin-Ying, Li Xiao-Dan
Institute of Cerebrovascular Diseases, Affiliated Hospital, Qingdao University Medical College, Qingdao Shandong 266003, China.
School of Chemistry, Chemical Engineering and Environmental Sciences, Qingdao University, Qingdao Shandong 266071, China.
Int J Mol Sci. 2012;13(3):3354-3365. doi: 10.3390/ijms13033354. Epub 2012 Mar 12.
Hypoglycemic effects and the use of kelp in diabetes mellitus (DM) model rats induced by alloxan were investigated. Sixty healthy male rats were used to establish DM models by injecting alloxan intraperitoneally. Kelp powder was added to the general forage for the rats. The levels of fasting blood glucose (FBG) were determined by an automatic blood glucose device. Electrochemiluminescence immunoassay was applied to determine the serum levels of insulin. The serum levels of malondialdehyde (MDA) were measured by thiobarbituric acid assay and nitric oxide (NO) by nitrate reductase assay. The activities of superoxide dismutase (SOD) were determined by xanthinoxidase assay and glutathione peroxidase (GSH-Px) by chemical colorimetry. The shape and structure of islet cells were observed with Hematine-Eosin staining, and the expression of superoxide dismutase (SOD) and inducible nitric oxide synthase (iNOS) in islet cells were detected by immunohistochemical assay. The results showed that the serum levels of insulin after treatment with kelp powder increased significantly compared to those in the DM-model group, while the FBG in the medium-high dose treated groups decreased significantly compared to those in the DM-model group (P < 0.05). The levels of MDA and NO in the kelp powder groups were lower than those in the DM-model group, while the activities of SOD and GSH-Px were higher than those in the DM-model group, of which a significant difference existed between the medium-high dose treated groups and the DM-model group (P < 0.05). The shape and structure of islet cells improved with the up-expressing SOD and down-expressing iNOS in the medium-high dose treated groups compared to those in the DM-model group (P < 0.05). There were no significant differences between the medium and high dose treated groups, all above indexes (P > 0.05). It is suggested that kelp might aid recovery of the the islet cell secreting function and reduce the level of FBG by an antioxidant effect.
研究了海带对四氧嘧啶诱导的糖尿病（DM）模型大鼠的降血糖作用及其应用。选用60只健康雄性大鼠，通过腹腔注射四氧嘧啶建立DM模型。将海带粉添加到大鼠的普通饲料中。采用自动血糖仪测定空腹血糖（FBG）水平。应用电化学发光免疫分析法测定血清胰岛素水平。采用硫代巴比妥酸法测定血清丙二醛（MDA）水平，采用硝酸还原酶法测定一氧化氮（NO）水平。采用黄嘌呤氧化酶法测定超氧化物歧化酶（SOD）活性，采用化学比色法测定谷胱甘肽过氧化物酶（GSH-Px）活性。采用苏木精-伊红染色观察胰岛细胞的形态和结构，采用免疫组织化学法检测胰岛细胞中超氧化物歧化酶（SOD）和诱导型一氧化氮合酶（iNOS）的表达。结果显示，与DM模型组相比，海带粉处理后血清胰岛素水平显著升高，而中高剂量处理组的FBG与DM模型组相比显著降低（P<0.05）。海带粉组的MDA和NO水平低于DM模型组，而SOD和GSH-Px活性高于DM模型组，其中中高剂量处理组与DM模型组之间存在显著差异（P<0.05）。与DM模型组相比，中高剂量处理组胰岛细胞的形态和结构有所改善，SOD表达上调，iNOS表达下调（P<0.05）。中剂量和高剂量处理组之间上述各项指标均无显著差异（P>0.05）。提示海带可能有助于胰岛细胞分泌功能的恢复，并通过抗氧化作用降低FBG水平。
