Cawston T, McLaughlan P, Coughlan R, Kyle V, Hazleman B
Rheumatology Research Unit, Addenbrooke's Hospital, Cambridge, U.K.
Biochim Biophys Acta. 1990 Jan 29;1033(1):96-102. doi: 10.1016/0304-4165(90)90200-g.
Samples of synovial fluids aspirated from patients with septic arthritis prior to the commencement of any treatment contained active metalloproteinases but no proteinase inhibitory activity. We therefore assayed these samples for proteinase-inhibitor complexes. Although no biologically active alpha 2-macroglobulin or tissue inhibitor of metalloproteinase (TIMP) was present in the fluids, immunoassay of the samples clearly showed that high molecular weight proteinase-TIMP complexes were present. It is proposed that high levels of active metalloproteinases are released from neutrophils into septic synovial fluids and that these proteinases complex all the available TIMP, forming metalloproteinase-TIMP complexes.
在任何治疗开始前从患有化脓性关节炎的患者身上抽取的滑液样本中含有活性金属蛋白酶，但没有蛋白酶抑制活性。因此，我们对这些样本进行了蛋白酶-抑制剂复合物检测。尽管这些液体中不存在具有生物活性的α2-巨球蛋白或金属蛋白酶组织抑制剂（TIMP），但对样本的免疫分析清楚地表明存在高分子量蛋白酶-TIMP复合物。有人提出，高水平的活性金属蛋白酶从中性粒细胞释放到化脓性滑液中，并且这些蛋白酶与所有可用的TIMP结合，形成金属蛋白酶-TIMP复合物。
