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影响植物乳杆菌423(从高粱啤酒中分离得到)所产细菌素植物乳杆菌素423吸附的参数。

Parameters affecting the adsorption of plantaricin 423, a bacteriocin produced by Lactobacillus plantarum 423 isolated from sorghum beer.

作者信息

Todorov Svetoslav D, Dicks Leon M T

机构信息

Department of Microbiology, University of Stellenbosch, Stellenbosch, South Africa.

出版信息

Biotechnol J. 2006 Apr;1(4):405-9. doi: 10.1002/biot.200500026.

Abstract

Plantaricin 423 is bactericidal to logarithmic and stationary-phase cells of Enterococcus sp. HKLHS and L. sakei DSM 20017. Detection of extracellular DNA and beta-galactosidase suggests that the mode of action is most probably by destabilizing of the cell membrane. Adsorption of plantaricin 423 to target cells ranged from 17% for Streptococcus caprinus ATCC 700066 to 67% for Lactobacillus plantarum LMG 13556, Lactobacillus curvatus DF38, Listeria innocua LMG 13568 and Lactobacillus sakei DSM 20017. Treatment of Enterococcus sp. HKLHS and L. sakei DSM 20017 with Triton X-100, Triton X-114 and chloroform increased the adsorption of plantaricin.

摘要

植物乳杆菌素423对肠球菌属HKLHS和清酒乳杆菌DSM 20017的对数期和稳定期细胞具有杀菌作用。细胞外DNA和β-半乳糖苷酶的检测表明,其作用模式很可能是通过破坏细胞膜的稳定性。植物乳杆菌素423对靶细胞的吸附率范围为:山羊链球菌ATCC 700066为17%,植物乳杆菌LMG 13556、弯曲乳杆菌DF38、无害李斯特菌LMG 13568和清酒乳杆菌DSM 20017为67%。用曲拉通X-100、曲拉通X-114和氯仿处理肠球菌属HKLHS和清酒乳杆菌DSM 20017可增加植物乳杆菌素的吸附。

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