Yasuda K, Shiozawa M, Aiso S, Taniguchi S, Yamashita S
Department of Anatomy, School of Medicine, Keio University, Tokyo, Japan.
J Histochem Cytochem. 1990 Mar;38(3):339-50. doi: 10.1177/38.3.1689336.
We studied in detail the distribution pattern of gamma-glutamyl transpeptidase (gamma-GT) in human pancreas, using the immunoperoxidase technique and a monoclonal antibody to human kidney gamma-GT. Positive reaction was confined exclusively to the luminal surface of the centroacinar cells and the epithelia of the intercalated, intralobular, and interlobular ducts. The immunoreaction was stronger in the intercalated and intralobular ducts than in the interlobular ducts. The acinar cells did not show any reaction. The islets of Langerhans were heavily surrounded by the ducts, and normal islet cells showed no reaction. The course of the ducts, from the acinar lumina to the interlobular ducts, was delineated by using reaction sites positive for gamma-GT as markers. The courses of the ducts, which comprise the pathway for pancreatic juice, were found to vary widely in their connections with each other, especially between the intralobular and interlobular ducts. At least three separate routes could be identified.
我们使用免疫过氧化物酶技术和针对人肾γ-谷氨酰转肽酶（γ-GT）的单克隆抗体，详细研究了γ-谷氨酰转肽酶在人胰腺中的分布模式。阳性反应仅局限于中央腺泡细胞的管腔表面以及闰管、小叶内导管和小叶间导管的上皮。闰管和小叶内导管中的免疫反应比小叶间导管中的更强。腺泡细胞未显示任何反应。胰岛被导管大量包围，正常胰岛细胞无反应。以γ-GT阳性反应位点为标记勾勒出从腺泡腔到小叶间导管的导管走行。发现构成胰液通路导管的走行在彼此连接上差异很大，尤其是在小叶内导管和小叶间导管之间。至少可识别出三条独立的路径。
