Aksoy Yilmaz, Aksoy Hülya, Altinkaynak Konca, Aydin Hasan Riza, Ozkan Asuman
Department of Urology, School of Medicine, Atatürk University, TR-25240 Erzurum, Turkey.
Prostaglandins Leukot Essent Fatty Acids. 2006 Aug;75(2):75-9. doi: 10.1016/j.plefa.2006.06.002. Epub 2006 Aug 8.
The lipid composition of spermatozoa plays an important role for successful fertilization.
In the present study, we analyzed the fatty acid (FA) composition of spermatozoa of normozoospermic, asthenozoospermic, oligozoospermic and oligoasthenozoospermic men.
Spermatozoa from asthenozoospermic (P<0.01), oligozoospermic (P<0.05) and oligoasthenozoospermic men (P<0.05) had lower levels of docosahexaenoic acid (22:6w3, DHA) than those from normozoospermic men. In oligozoospermic and asthenozoospermic men, spermatozoa 18:0 content was higher than that of normozoospermics (P<0.01 and P<0.001, respectively). 18:1w9 was higher in oligoasthenozoospermic and oligozoospermic samples when compared with normozoospermic samples (P<0.05 for both). While from the point of view of total w6 FAs there was no significant difference among the groups, the w6/w3 ratio was significantly higher in asthenozoospermic samples than in normozoospermic samples (P<0.05). Monounsaturated fatty acids (MFA) were higher in oligozoospermic samples (P<0.05) than in normozoospermic samples, polyunsaturated fatty acids (PUFA) were lower in asthenozoospermic (P<0.01), oligoasthenozoospermic (P<0.05) and oligozoospermic samples (P<0.05) than in normozoospermic samples. Saturated fatty acids (SFA) were significantly higher in asthenozoospermic (P<0.01) and oligozoospermic samples (P<0.05) compared with normozoospermic samples. In correlation analysis, there were significant positive correlations between DHA with sperm motility (r=0.53), sperm concentration (r=0.36) and normal sperm morphology (r=0.30). In addition, there were significant correlations between PUFA with sperm motility (r=0.50), sperm concentration (r=0.35), and normal sperm morphology (r=0.28), and between w6/w3 with sperm motility (r=-0.47), sperm concentration (r=-0.27), and normal sperm morphology(r=-0.24).
These suggest that decreased DHA and PUFA, and increased w6/w3 in spermatozoa may be related to infertility in oligo- and/or asthenozoospermic men.
精子的脂质组成对成功受精起着重要作用。
在本研究中，我们分析了正常精子症、弱精子症、少精子症和少弱精子症男性精子的脂肪酸（FA）组成。
弱精子症（P<0.01）、少精子症（P<0.05）和少弱精子症男性（P<0.05）的精子中二十二碳六烯酸（22:6w3，DHA）水平低于正常精子症男性。在少精子症和弱精子症男性中，精子18:0含量高于正常精子症男性（分别为P<0.01和P<0.001）。与正常精子症样本相比，少弱精子症和少精子症样本中的18:1w9更高（两者均为P<0.05）。虽然从总w6脂肪酸的角度来看，各组之间没有显著差异，但弱精子症样本中的w6/w3比值显著高于正常精子症样本（P<0.05）。少精子症样本中的单不饱和脂肪酸（MFA）高于正常精子症样本（P<0.05），弱精子症（P<0.01）、少弱精子症（P<0.05）和少精子症样本中的多不饱和脂肪酸（PUFA）低于正常精子症样本（P<0.05）。与正常精子症样本相比，弱精子症（P<0.01）和少精子症样本中的饱和脂肪酸（SFA）显著更高（P<0.05）。在相关性分析中，DHA与精子活力（r=0.53）、精子浓度（r=0.36）和正常精子形态（r=0.30）之间存在显著正相关。此外，PUFA与精子活力（r=0.50）、精子浓度（r=0.35）和正常精子形态（r=0.28）之间，以及w6/w3与精子活力（r=-0.47）、精子浓度（r=-0.27）和正常精子形态（r=-0.24）之间存在显著相关性。
这些结果表明，精子中DHA和PUFA的减少以及w6/w3的增加可能与少精子症和/或弱精子症男性的不育有关。
