Voisset Cécile, de Beeck Anne Op, Horellou Pauline, Dreux Marlène, Gustot Thierry, Duverlie Gilles, Cosset François-Loic, Vu-Dac Ngoc, Dubuisson Jean
CNRS, Institut de Biologie de Lille (UMR8161), Institut Pasteur de Lille, 1 rue Calmette, BP447, 59021 Lille cedex, France.
Free University of Brussels, Faculty of Medicine, Laboratory of Molecular Virology, Brussels, Belgium.
J Gen Virol. 2006 Sep;87(Pt 9):2577-2581. doi: 10.1099/vir.0.81932-0.
The neutralizing activity of anti-hepatitis C virus (HCV) antibodies is attenuated by a factor present in human sera, which has been proposed to be high-density lipoproteins (HDLs). HDLs have also been shown to facilitate the entry of HCV pseudoparticles (HCVpp) into target cells. Here, the aim of the study was to determine whether HDL-mediated facilitation of HCVpp and infectious HCV (HCVcc) entry and attenuation of neutralization are two related phenomena. The data indicated that HDLs attenuate neutralization at a constant rate. In addition, as for HDL-mediated facilitation of HCVpp entry, attenuation of neutralization depended on the expression of the scavenger receptor BI (SR-BI) and its selective lipid-uptake function. Finally, kinetic experiments showed that HDL-mediated facilitation of HCVpp entry is more rapid than virus neutralization. Altogether, these observations indicate that HCV is exploiting the physiological activity of SR-BI for promoting its entry into target cells, which consequently also protects the virus against neutralizing antibodies.
人血清中存在的一种因子会减弱抗丙型肝炎病毒（HCV）抗体的中和活性，该因子被认为是高密度脂蛋白（HDL）。HDL还被证明可促进HCV假病毒颗粒（HCVpp）进入靶细胞。在此，本研究的目的是确定HDL介导的HCVpp和传染性HCV（HCVcc）进入的促进作用以及中和作用的减弱是否为两个相关现象。数据表明，HDL以恒定速率减弱中和作用。此外，至于HDL介导的HCVpp进入的促进作用，中和作用的减弱取决于清道夫受体BI（SR-BI）的表达及其选择性脂质摄取功能。最后，动力学实验表明，HDL介导的HCVpp进入的促进作用比病毒中和作用更快。总之，这些观察结果表明，HCV正在利用SR-BI的生理活性来促进其进入靶细胞，从而也保护病毒免受中和抗体的作用。
