Sandvik A K, Waldum H L
Department of Medicine, University of Trondheim, Norway.
Life Sci. 1990;46(6):453-9. doi: 10.1016/0024-3205(90)90089-a.
Acid secretion and histamine release from the totally isolated vascularly perfused rat stomach are sensitive parameters for the biological effects of gastrin. By optimalizing the Ca2+ concentration of the vascular perfusate the sensitivity for stimulation of acid secretion was improved from 65 to 16 pmol/l gastrin 1-17 (G-17). Measurement of venous histamine before and after gastrin in each stomach preparation excluded inter-stomach variability and improved the sensitivity to 2 pmol/l G-17. This is the most sensitive gastrin bioassay reported, with a detection limit comparable to that of radioimmunoassay.
从完全分离并经血管灌注的大鼠胃中分泌的胃酸以及组胺释放，是胃泌素生物学效应的敏感指标。通过优化血管灌注液中的钙离子浓度，胃酸分泌刺激的敏感性从65皮摩尔/升胃泌素1-17（G-17）提高到了16皮摩尔/升。在每个胃标本中测量胃泌素给药前后的静脉组胺，排除了不同胃之间的差异，并将敏感性提高到了2皮摩尔/升G-17。这是所报道的最敏感的胃泌素生物测定法，其检测限与放射免疫测定法相当。
