使用离体血管灌注大鼠胃进行胃泌素生物测定。一种新的、简化且灵敏的方法。
Bioassay of gastrin using the isolated vascularly perfused rat stomach. A new, simplified and sensitive method.
作者信息
Mårvik R, Sandvik A K, Waldum H L
机构信息
Department of Surgery, Trondheim University Hospital, Norway.
出版信息
Acta Physiol Scand. 1995 Nov;155(3):323-7. doi: 10.1111/j.1748-1716.1995.tb09980.x.
Radioimmunoassays are sensitive and specific methods for measurement of the concentrations of regulatory peptides. However, aspects of physiological, pathophysiological and pharmacological research require knowledge about the biological activity which does not necessarily vary concomitantly with immunological activity. The present work describes a simplified bioassay for gastrin based on the gastric histamine releasing properties of this peptide, using an isolated vascularly perfused rat stomach preparation with a crystalline perfusate and a specific radioimmunoassay for histamine. The establishment of a dose-response curve is described, as well as the utilization of the bioassay on sera from patients with hypergastrinaemia. The method is sensitive for gastrin in the low (4 pmol L-1) picomolar range.
放射免疫测定法是测量调节肽浓度的灵敏且特异的方法。然而，生理、病理生理和药理研究的某些方面需要了解生物活性，而生物活性不一定与免疫活性同步变化。本研究描述了一种基于胃泌素释放组胺特性的简化生物测定法，该方法使用分离的经血管灌注的大鼠胃制备物，灌注液为晶体，并采用组胺特异性放射免疫测定法。文中描述了剂量反应曲线的建立，以及该生物测定法在高胃泌素血症患者血清中的应用。该方法对低皮摩尔范围（4 pmol L-1）的胃泌素敏感。
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