Widyma Agnieszka, Piekarska Jolanta
Instytut Parazytologii im. Witolda Stefańskiego PAN, ul. Norwida 31, 50-375 Wrocław.
Wiad Parazytol. 2002;48(4):413-7.
The apoptosis in the course of experimental infection with T. pseudospiralis in mice. The level of apoptic cells in the lamina propria of the mucosa of the jejunum and in the masseter muscle in the mice infected with 200 larvae T. pseudospiralis was observed. Cryostat preparations made from jejunum and muscle samples on 7, 14, 21, 28, 60, 180 and 360 day post infection (dpi), were examined employing the TUNNEL method with the use of "In situ Cell Death Detection KIT Fluorescein" of Roche. The number of apoptic cells in the lamina propria of jejunum started to increase as early as on 7 dpi. The highest number of the apoptic cells, on an average - 56,4 in the crypta-villus unit, was recorded on 28 dpi, whereas the return to the standard level took place only 360 dpi. The level of the apoptic cells in the masseter muscle was the highest on 28 and 60 dpi, which constituted some 30% of all the cells of infiltration.
小鼠感染伪旋毛虫实验过程中的细胞凋亡。观察了感染200条伪旋毛虫幼虫的小鼠空肠黏膜固有层和咬肌中凋亡细胞的水平。在感染后第7、14、21、28、60、180和360天，从空肠和肌肉样本制备冰冻切片，采用罗氏公司的“原位细胞死亡检测试剂盒荧光素”，运用TUNNEL法进行检测。空肠固有层中凋亡细胞数量早在感染后第7天就开始增加。在感染后第28天，隐窝-绒毛单位中凋亡细胞的平均数量最高，为56.4个，而直到感染后360天才恢复到正常水平。咬肌中凋亡细胞水平在感染后第28天和60天最高，约占所有浸润细胞的30%。
