Karmańska K, Houszka M, Widyma A, Stefaniak E
W. Stefański Institute of Parasitology, Polish Academy of Science, Wrocław, Poland.
Wiad Parazytol. 1997;43(3):251-6.
In the course of trichinellosis in mice the numerous cells inside larval capsules were observed. Beginning on 14 day post infection (dpi) they were seen in about 7% of infected muscles fibers but at 21 dpi the percentage of capsules with the cells amounted to 77. In the next stage of infection the number of capsules containing cells continued to increase and reach almost 100% at 60 dpi. The identification of the cells was carried out by the indirect immunoenzymatic method using anti-CD4+, anti-CD8+ and anti-macrophage (Mac-1 antigen) monoclonal antibodies. In course of observation the level of CD4+ cells decreased from 11.3 to 0.7, of CD8+ from 17.7 to 3.0 and of macrophages from 71.0 to 23.1%. The most numerous CD4+ cells were seen at 14 dpi while the CD8+ and macrophages at 21 dpi. Beginning on 28 dpi negatively reacting cells inside larval capsules were also observed. The number of these cells increased from 23.8 to 73.2% at 60 dpi and their identification requires further investigations. The role and the function of all these cells are discussed.
在小鼠旋毛虫病病程中，观察到幼虫包囊内有大量细胞。感染后14天（dpi）开始，在约7%的受感染肌纤维中可见到这些细胞，但在21 dpi时，含有这些细胞的包囊百分比达77%。在感染的下一阶段，含有细胞的包囊数量持续增加，在60 dpi时几乎达到100%。使用抗CD4 +、抗CD8 +和抗巨噬细胞（Mac - 1抗原）单克隆抗体，通过间接免疫酶法对细胞进行鉴定。在观察过程中，CD4 +细胞水平从11.3%降至0.7%，CD8 +细胞从17.7%降至3.0%，巨噬细胞从71.0%降至23.1%。在14 dpi时可见到数量最多的CD4 +细胞，而CD8 +细胞和巨噬细胞在21 dpi时最多。从28 dpi开始，还观察到幼虫包囊内有阴性反应细胞。这些细胞的数量在60 dpi时从23.8%增加到73.2%，其鉴定需要进一步研究。本文讨论了所有这些细胞的作用和功能。
