Abhold R H, Bowker R M
Department of Biology, California State University at Fresno 93740-0073.
Neurosci Lett. 1990 Jan 1;108(1-2):231-6. doi: 10.1016/0304-3940(90)90736-s.
Bipolar concentric stimulating electrodes were placed medially within the nucleus raphe magnus and laterally within the nucleus gigantocellularis. The levels of norepinephrine (NE), serotonin (5-HT), and 5-hydroxyindolacetic acid (5-HIAA) within the dorsal horn of the spinal cord were measured before and after electrical stimulation of these brainstem nuclei using in vivo dialysis coupled with high pressure liquid chromatography and electrochemical detection. Stimulation medially significantly increased the levels of all three amines. While simulation laterally also increased NE, both 5-HT and 5-HIAA were significantly reduced. The relevance of these findings to descending modulation of ascending nociceptive neural activity within the dorsal horn is discussed.
双极同心刺激电极被置于中缝大核内侧和巨细胞网状核外侧。在对这些脑干核进行电刺激前后，采用体内透析结合高压液相色谱和电化学检测法，测量脊髓背角中去甲肾上腺素（NE）、5-羟色胺（5-HT）和5-羟吲哚乙酸（5-HIAA）的水平。内侧刺激显著增加了所有三种胺类的水平。而外侧刺激虽然也增加了NE，但5-HT和5-HIAA均显著降低。本文讨论了这些发现与脊髓背角内伤害性神经活动上行调制的下行调节的相关性。
