Colado M I, Arnedo A, Peralta E, Del Río J
Department of Neuropharmacology, Cajal Institute, C.S.I.C., Madrid, Spain.
Neurosci Lett. 1988 May 3;87(3):302-6. doi: 10.1016/0304-3940(88)90466-1.
In the rat, unilateral dorsal cervicothoracic rhizotomy (C5-T2) resulted in autotomy of the ipsilateral limb. The onset of self-mutilation was variable and attained the maximum degree 8-9 weeks after the dorsal root section. Fifteen and 60 days after the lesion the monoamines dopamine (DA), noradrenaline (NA) and serotonin (5-HT) as well as the 5-HT metabolite 5-hydroxyindoleacetic acid (5-HIAA) were measured by high performance liquid chromatography at different levels of the spinal cord. The lesion induced a significant decrease in the spinal levels of DA and NA, both ipsilateral and contralateral to the lesion, not only in the deafferented region but also from T3 downwards. The changes in DA levels were more marked and of earlier onset than those of NA. The decrease in 5-HT and its metabolite 5-HIAA was only observed in the more caudal regions of the non-deafferented ipsilateral cord. The more conspicuous lessening in spinal monoamine levels coincided approximately with the maximum intensity of autotomy. The present results suggest that dorsal rhizotomy induces distant changes in the brainstem nuclei where descending aminergic pathways originate.
在大鼠中，单侧颈胸段背根切断术（C5-T2）导致同侧肢体自残。自残的发作时间不一，在背根切断术后8-9周达到最大程度。在损伤后15天和60天，通过高效液相色谱法在脊髓的不同水平测量单胺类物质多巴胺（DA）、去甲肾上腺素（NA）和5-羟色胺（5-HT）以及5-HT代谢产物5-羟吲哚乙酸（5-HIAA）。该损伤不仅在去传入区域，而且在T3以下水平，导致损伤同侧和对侧脊髓中DA和NA水平显著降低。DA水平的变化比NA更明显且发作更早。5-HT及其代谢产物5-HIAA的降低仅在未去传入的同侧脊髓更靠尾端的区域观察到。脊髓单胺水平更明显的降低大约与自残的最大强度同时出现。目前的结果表明，背根切断术会引起脑干核团的远距离变化，下行胺能通路起源于此。
