培养介质的pH值对简单异尖线虫Ⅲ期幼虫存活及发育的影响
Influence of the pH of the cultivation medium on survival and development of stage III larvae of Anisakis simplex.
作者信息
Dziekońska-Rynko J, Rokicki J, Jabłonowski Z, Białowas K
机构信息
Department of Parasitology, University of Warmia and Mazury, 14 Zołnierska Street, 10-561 Olsztyn.
出版信息
Wiad Parazytol. 2001;47(3):317-22.
The cultivation was done on Eagle's media as well as Eagle's media enriched with the supplement of 10 and 20% of bovine serum. In the media without serum the larvae lived significantly shorter and did not get through molting. They lived the longest and came through molting the fastest in the media with pH 2.0 supplemented with 20% SB. In the media with the pH 4.0 and 7.3 the larvae lived significantly shorter and only a small proportion reached stage IV.
培养是在伊格尔培养基以及添加了10%和20%牛血清补充剂的伊格尔培养基上进行的。在无血清的培养基中，幼虫存活时间显著缩短，且无法完成蜕皮。在添加了20%牛血清、pH值为2.0的培养基中，它们存活时间最长，蜕皮速度最快。在pH值为4.0和7.3的培养基中，幼虫存活时间显著缩短，只有一小部分能发育到第四阶段。
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