Franke E D, Riberu W, Wiady I
U.S. Naval Medical Research Unit No. 2, Jakarta Detachment, APO San Francisco, California 96356.
J Parasitol. 1990 Apr;76(2):262-5.
Third-stage larvae (L3) of Wuchereria bancrofti molt to the fourth stage in an in vitro culture medium composed of NCTC 135 and Iscove's modified Dulbecco's medium (1:1; v/v) supplemented with 10% human serum and a mixture of anti-bacterial and anti-mycotic agents. In the present investigation this culture medium was used to examine the effects of different concentrations of human serum, medium supplements, and serum replacements on larval growth, development, and molting. Several medium supplements and serum replacements were evaluated including hemin, Nutridoma, and a mixture of soybean lipids, bovine serum albumin, and transferrin. The supplements tested could not support larval growth and development in the absence of serum and they did not have an enhancing effect on larval growth and development in combination with human serum. A medium supplement of 30% human serum resulted in molting of 80-94% of L3s and optimum growth to the mid to late fourth stage. This culture system provides an excellent alternative to experimentally infected animals as a source of larvae undergoing the third molt and fourth-stage larvae for screening potential anti-filarial compounds and for immunologic and biochemical studies.
班氏吴策线虫的三期幼虫（L3）在由NCTC 135和伊斯科夫改良的杜尔贝科培养基（1:1；v/v）组成的体外培养基中蜕皮至第四期，该培养基补充有10%的人血清以及抗菌和抗真菌剂混合物。在本研究中，使用该培养基来检测不同浓度的人血清、培养基补充剂和血清替代物对幼虫生长、发育和蜕皮的影响。评估了几种培养基补充剂和血清替代物，包括血红素、纽蛋白以及大豆脂质、牛血清白蛋白和转铁蛋白的混合物。所测试的补充剂在无血清时不能支持幼虫生长和发育，并且与人类血清联合使用时对幼虫生长和发育没有增强作用。30%人血清的培养基补充剂导致80 - 94%的L3蜕皮，并实现向第四期中后期的最佳生长。该培养系统为实验感染动物提供了一个极好的替代方案，作为经历第三次蜕皮的幼虫和第四期幼虫的来源，用于筛选潜在的抗丝虫化合物以及进行免疫学和生物化学研究。
