Zarnowska-Prymek H
Klinika Chorób Odzwierzecych i Tropikaknych AM, 01-201 Warszawa, ul. Wolska 37.
Wiad Parazytol. 2001;47(3):489-96.
The aim of the study was to enhance specificity of immunological diagnosis in toxocarosis. The investigations concern: detection of circulating parasite antigens using dot-ELISA technique and detection of specific IgG antigodies using ELISA test with low molecular weight (< or = 30 kD) excretory-secretory (ES) larval T. canis antigens. The circulating antigens were investigated in 60 sera and 20 anterior chamber fluids from children with ocular form of toxocarosis. These antigens were present only in 3 sera and 2 anterior chamber fluids in children with active intraocular inflamation and fluid antibodies titers 1:5120 and 1:20 000. Comparative study with different ELISA tests indicated that use of low molecular weight antigens increase specificity of reaction.
本研究的目的是提高弓蛔虫病免疫诊断的特异性。研究内容包括：采用斑点酶联免疫吸附测定（dot-ELISA）技术检测循环中的寄生虫抗原，以及使用低分子量（≤30 kD）犬弓首线虫幼虫排泄分泌（ES）抗原的酶联免疫吸附测定（ELISA）试验检测特异性IgG抗体。对60份血清和20份来自患眼型弓蛔虫病儿童的前房液进行了循环抗原检测。这些抗原仅在眼内有活动性炎症且抗体滴度分别为1:5120和1:20 000的3份血清和2份前房液中存在。与不同ELISA试验的对比研究表明，使用低分子量抗原可提高反应的特异性。
