[单纯疱疹病毒1型重组型特异性表位的多拷贝表达及免疫反应性]
[Expression of multi-copy and immuno-reactivity of recombinant type-specific epitope of herpes simplex virus type 1].
作者信息
Ji Xiao-wei, Mao Xu-hu, Zou Quan-ming, Yu Qing-tan, Zhao Li-li
机构信息
Department of Clinical Microbiology and Immunology, the Third Military Medical University and Chongqing Research Center of Biopharmaceutical Engineering Technology, Chongqing 400038, China.
出版信息
Zhonghua Nan Ke Xue. 2006 Jul;12(7):579-82.
OBJECTIVE
To express a multi-copy specific epitope recombinant protein of herpes simplex virus type 1 (HSV-1) with immuno-reactivity.
METHODS
Multi-copy genes with a specific epitope of HSV-1-glycoprotein G 112-127 were constructed by DNA recombination and cloned in E. coli JM109 pGEM-5Zf. The positive recombinants were determined by SDS-PAGE and Western blotting.
RESULTS
The recombinants with 4, 8, 16 and 32 copies of gG 112-127 were obtained. The 8-copy recombinant was expressed by 17.5%, mainly as inclusion body. And it reacted with antiserum HSV-1, but not with antiserum HSV-2.
CONCLUSION
The HSV-1-gG112-127 recombinant could be used to distinguish HSV-1 and HSV-2 in ELISA.
目的
表达具有免疫反应性的1型单纯疱疹病毒（HSV-1）多拷贝特异性表位重组蛋白。
方法
通过DNA重组构建具有HSV-1糖蛋白G 112-127特异性表位的多拷贝基因，并克隆到大肠杆菌JM109 pGEM-5Zf中。通过SDS-PAGE和蛋白质免疫印迹法鉴定阳性重组体。
结果
获得了具有4、8、16和32个拷贝gG 112-127的重组体。8拷贝重组体的表达量为17.5%，主要以包涵体形式存在。它能与HSV-1抗血清反应，但不与HSV-2抗血清反应。
结论
HSV-1-gG112-127重组体可用于酶联免疫吸附测定（ELISA）中区分HSV-1和HSV-2。
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