Palma-Carlos G, Simoes M H, Palma-Carlos M L
Inst. Immunologia, C.H.I.U.L. Fac. Medicina, Lisboa, Portugal.
Allerg Immunol (Paris). 1990 Jan;22(1):5, 7-9.
Human Basophil Degranulation Test (HBDT) has been employed for the research of Histamine Releasing Factor (HRF) from macrophages in asthma. Macrophages have been obtained from broncho-alveolar lavages, incubated with specific allergens and the products of this stimulation added to HBDT preparations. The production of an active HRF has been observed only in macrophages from atopic patients. The effect of this HRF seems to be IgE dependent, the degranulation of human basophils being in general significant only for basophils of atopic patients.
人嗜碱性粒细胞脱颗粒试验（HBDT）已被用于研究哮喘中巨噬细胞释放组胺因子（HRF）。巨噬细胞取自支气管肺泡灌洗物，与特定过敏原一起孵育，然后将这种刺激的产物添加到HBDT制剂中。仅在特应性患者的巨噬细胞中观察到活性HRF的产生。这种HRF的作用似乎依赖于IgE，一般来说，人嗜碱性粒细胞的脱颗粒仅对特应性患者的嗜碱性粒细胞有显著影响。
