Joseph M, Tonnel A B, Capron A, Voisin C
Clin Exp Immunol. 1980 May;40(2):416-22.
Human alveolar macrophages specifically released lysosomal beta-glucuronidase and neutral proteases when successively incubated with IgE, and then, for 30 min, with anti-IgE. Superoxide anion O2- generation was obtained when anti-IgE-opsonized zymosan was added to IgE-incubated cells. Macrophages from smokers excreted twice as much enzymes and superoxide as cells from non-smokers. It was possible to induce the specific release of beta-glucuronidase with normal alveolar macrophages successively incubated with the serum of patients allergic to house dust or to grass pollen and then with the specific allergen. This characteristic opens the field to a direct test for allergic sera by analogy with the allergen-induced degranulation test of sensitized basophils.
当人肺泡巨噬细胞先后与IgE孵育，然后再与抗IgE孵育30分钟时，会特异性释放溶酶体β-葡萄糖醛酸酶和中性蛋白酶。当将抗IgE调理的酵母聚糖添加到经IgE孵育的细胞中时，会产生超氧阴离子O2-。吸烟者的巨噬细胞分泌的酶和超氧化物是非吸烟者细胞的两倍。用正常肺泡巨噬细胞先后与对屋尘或草花粉过敏患者的血清孵育，然后再与特异性变应原孵育，有可能诱导β-葡萄糖醛酸酶的特异性释放。这一特性为通过类似于致敏嗜碱性粒细胞的变应原诱导脱颗粒试验直接检测过敏血清开辟了领域。
