Xiao Ning, Nakao Minoru, Qiu Jiamin, Budke Christine M, Giraudoux Patrick, Craig Philip S, Ito Akira
Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan.
Am J Trop Med Hyg. 2006 Aug;75(2):292-4.
The eastern Qinghai-Tibet plateau of China is a highly endemic region of echinococcosis where Echinococcus granulosus sensu stricto (sheep strain), Echinococcus multilocularis, and Echinococcus shiquicus are distributed sympatrically. We developed a polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) method for the identification of the three species in this region. The PCR-RFLP showed the dual infection of animals with different Echinococcus spp. The first case was a domestic dog concurrently infected with adults of E. granulosus and E. multilocularis. The second case was a plateau pika (Ochotona curzoniae) harboring metacestodes of E. multilocularis and E. shiquicus in the liver. The high susceptibility of some mammalian hosts to the parasites and the high prevalence of the three co-endemic species probably increase the chance of mixed infections in the eastern Tibetan plateau.
中国青藏高原东部是棘球蚴病的高度流行区，细粒棘球绦虫（绵羊株）、多房棘球绦虫和石渠棘球绦虫在该地区同域分布。我们开发了一种基于聚合酶链反应的限制性片段长度多态性（PCR-RFLP）方法，用于鉴定该地区的这三种物种。PCR-RFLP显示动物存在不同棘球绦虫物种的双重感染。第一例是一只家犬同时感染了细粒棘球绦虫和多房棘球绦虫的成虫。第二例是一只高原鼠兔（高原鼠兔）肝脏中寄生有多房棘球绦虫和石渠棘球绦虫的中绦期幼虫。一些哺乳动物宿主对这些寄生虫的高易感性以及这三种共流行物种的高流行率可能增加了青藏高原东部混合感染的机会。
