Baganha M F, Pêgo A, Lima M A, Gaspar E V, Cordeiro A R
Centro de Pneumologia da Universidade de Coimbra, Portugal.
Chest. 1990 Mar;97(3):605-10. doi: 10.1378/chest.97.3.605.
This study attempts to correlate levels of ADA in tuberculous and neoplastic pleural exudates with the different immunologic cellular expressions that follow these clinical situations. Seventy-three patients with pleural effusion were studied in order to assess ADA activity (pleural and serum); in 25 of them, a study of delayed cellular immunity (pleural and sanguineous) was performed through B, CD3, CD4, and CD8 lymphocytic populations. The activity of ADA was determined, and the study of lymphocytic populations was made through the use of monoclonal antibodies. The data obtained showed the following: levels of ADA were significantly (p less than 0.0005) higher in the pleural fluid and the serum of tuberculous effusions compared to neoplastic effusions; percentages of CD3 and CD4 T-cells were significantly (p less than 0.05 and p less than 0.0005, respectively) greater in tuberculous effusions. The statistical study of the levels of ADA activity and the percentage of CD4 T-cells in pleural exudates produced a significant regression curve (r = 0.612 and p less than 0.0001) which showed a positive correlation between these two parameters. The pathogenic implications of these results suggest the possibility that ADA could be a new marker of cell-mediated immune activity.
本研究试图将结核性和肿瘤性胸腔积液中ADA的水平与这些临床情况后的不同免疫细胞表达相关联。为评估ADA活性（胸腔积液和血清），对73例胸腔积液患者进行了研究；其中25例通过B、CD3、CD4和CD8淋巴细胞群体对迟发型细胞免疫（胸腔积液和血液）进行了研究。测定了ADA活性，并通过使用单克隆抗体对淋巴细胞群体进行了研究。获得的数据显示如下：与肿瘤性胸腔积液相比，结核性胸腔积液的胸腔积液和血清中ADA水平显著更高（p＜0.0005）；结核性胸腔积液中CD3和CD4 T细胞的百分比显著更高（分别为p＜0.05和p＜0.0005）。对胸腔积液中ADA活性水平和CD4 T细胞百分比的统计学研究产生了一条显著的回归曲线（r = 0.612，p＜0.0001），表明这两个参数之间呈正相关。这些结果的致病意义提示ADA可能是细胞介导免疫活性的一种新标志物。
